Question

adapter trimming using trimmomatic

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4.0 years ago

GR ▴ 400

Hi All,

I ran fastqc on my chipseq dataset and a redflag is raised for overrepresented sequences.

29% of this sequence- GATCGGAAGAGCACACGTCTGAACTCCAGTCACACA (possible source- Trueseq adapter)

I ran trimmomatic for both Trueseq2 and Trueseq3 but both don't seem to trim anything. Any suggestions?

Thanks, Ritu

adapter-trimming next-gen-sequencing • 1.3k views

ADD COMMENT • link updated 21 months ago by Ram 43k • written 4.0 years ago by GR ▴ 400

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Provide command lines you used. You could also try bbduk.sh from BBMap suite which provides a common adapters file you can use in recources folder in software distribution. A guide is available.

ADD REPLY • link 4.0 years ago by GenoMax 141k

score 0 · Answer 1 · 2021-10-14

Maybe my answer is not actual now, but: you can use a custom adapter sequence in trimmomatic. Here are instructions from the trimmomatic manual:

ILLUMINACLIP:<fastaWithAdaptersEtc>:<seed mismatches>:<palindrome clip threshold>:<simple clip threshold>

fastaWithAdaptersEtc: specifies the path to a fasta file containing all the adapters, PCR sequences, etc. The naming of the various sequences within this file determines how they are used. See the section below or use one of the provided adapter files

Just create fasta file with an adapter or other overrepresented sequence and feed it to trimmomatic.