Demultiplexing with Linux

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2.7 years ago

Nic • 0

Hi,

I am currently finishing my Master's degree and I am relatively new to this subject. I would like to ask if anyone knows how to do demultiplexing via linux. Specifically I have found this example (https://astrobiomike.github.io/amplicon/demultiplexing) which I was able to implement by downloading all the programs I need to work with, however I can't work with my own files and sequences. I would greatly appreciate it if someone could help me.

Thank you,

Nikos

Demultiplexing Linux • 1.2k views

ADD COMMENT • link updated 2.7 years ago by cpad0112 21k • written 2.7 years ago by Nic • 0

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I can't work with my own files and sequences

Are you getting errors?

ADD REPLY • link 2.7 years ago by GenoMax 141k

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Yes, I think I'm making mistakes, so I'm getting a lot of erros. I don't know how to insert my file into the above example to make it work.

ADD REPLY • link 2.7 years ago by Nic • 0

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You will need to make a mapping file as shown in the example. That appears to be a tab limited file. Make a note of the column headers and make sure you format your file right.

ADD REPLY • link 2.7 years ago by GenoMax 141k

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Please post example data, the function/command you are using and errors you are getting. That would help forum members to address the errors.

ADD REPLY • link 2.7 years ago by cpad0112 21k

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