Should I expect high duplicate read frequency in my scRNA fastq's?
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2.6 years ago
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Hi there, I'm new to scRNA-seq data and I am looking at a multiqc report for some of my samples. The screenshot below shows the sequence counts for my fastq's. My question is, should I expect such a high frequency of duplicate reads? Any help appreciated.

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scRNA-seq fastqc multiqc • 636 views
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We'll, this is before any UMI duplicate removal, right? Is your prep biased towards one end?

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