exceRpt miRNA pipeline for paired-end RNA-seq data

Question

[Hirofumi]

Hello.
I am trying to count miRNAs using exceRpt pipeline (using Genbree, not Docker because it dosen't work).
I could count miRNA expressions in single-read RNA-seq data.

Do you know how to count paired-end RNA-seq data?

Thank you for reading!

[Buffo]

miRNAs are really short sequences, mature mirnas are ~22 nucleotides long, precursors are ~70pb. I don't think that it is possible to count miRNAs from paired-end RNAseq.

[Hirofumi]

Thank you for reply, Buffo!
I was wondering if it could be analyzed using NCBI GEO's paired-end public data.

I got it now.