id1 ="HWI-EAS380:8:1:16:830/1"

seq1 ="AGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTTCTCT
       AAAAACCATTTTTCGTCCCCTTCGGGGCGGTGGTCTATAGTGTTATTAATATCAA
       GTTGGGGGAGCACATTGTAGCATTG"

qual1="abbbbbaab`abaabbaabaaaab^E^``^aaabaa_\_abaaaaaaaa`aaaa`
       Z^`^^aaaaaaa`aa^aaa``_aa_aaaaaaaaaaa`aaaa`aaaaaabaaabba
       aaaaaaaaaaa_baaaabbbbbaba"

assume these are fastq-illumina quality scores and the sequence is unambiguous dna

I want to create a SeqRecord object from this. Thanks.

This answer is a bit of a cheat, but currently Biopython doesn't expose the FASTQ quality string decoding functionality in the public API:

from Bio import SeqIO
from StringIO import StringIO
fastq_string = "@%s\n%s\n+\n%s\n" % (id1, seq1, qual1)
record = SeqIO.read(StringIO(fastq_string), "fastq-illumina")

Why are you trying to do this?

id1 ="HWI-EAS380:8:1:16:830/1" >>> seq1 ="AGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTT" >>> from Bio.SeqRecord import SeqRecord >>> from Bio.Seq import Seq >>> sr = SeqRecord(Seq(seq1), id1, '', '') >>> sr SeqRecord(seq=Seq('AGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTT', Alphabet()), id='HWI-EAS380:8:1:16:830/1', name='', description='', dbxrefs=[])