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110,509 results • Page
2 of 2211
Sort: Rank
Rank
Views
Votes
Replies
1
vote
0
replies
109
views
How to predict gene amplification from RNAseq and ATACseq data?
amplification
ATAC
1 day ago by
Dan
▴ 120
6
votes
7
replies
350
views
NCBI API, Perl
API
NCBI
updated 1 day ago by
GenoMax
129k • written 1 day ago by
alessandro.alma00
• 0
1
vote
2
replies
175
views
hisat2 Error 137
hisat2
RNA-seq
1 day ago by
Forough
▴ 10
0
votes
0
replies
110
views
Error occured at the PHG imputation step: allele in genotype not in the variant context
PHG
Imputation
PracticalHaplotypeGraph
Haplotype
1 day ago by
xeod21
• 0
1
vote
0
replies
127
views
Herald:
The Biostar Herald for Monday, June 05, 2023
herald
1 day ago by
Biostar
1.8k
2
votes
1
reply
144
views
Is RNA-Seq well suited for studying pseudogenes and lncRNA?
rna
ncRNA
transcriptome
pseudogene
rna-seq
updated 1 day ago by
rpolicastro
12k • written 1 day ago by
Kermit
▴ 80
2
votes
5
replies
288
views
Embryo transcriptome
database
transcriptome
SRA
updated 1 day ago by
ATpoint
72k • written 1 day ago by
firefox91
• 0
1
vote
3
replies
182
views
Obtain a --gene-trans-map file after rnaSPAdes
rnaSPAdes
Trinity
1 day ago by
UserA
• 0
1
vote
0
replies
118
views
Tutorial:
Get a list of genes associated with a drug in 1 min
drug
genes
drug-targets
updated 1 day ago by
Ram
39k • written 1 day ago by
Coremine Medical
▴ 10
2
votes
3
replies
282
views
Getting species names and taxa id from assembly accession number
efetch
biopython
entrez
updated 1 day ago by
MirianT_NCBI
▴ 600 • written 2 days ago by
kwhiggins27
▴ 10
0
votes
0
replies
86
views
How to extract only neutral markers from low-coverage whole genome sequencing?
low-coverage
whole-genome-sequencing
updated 1 day ago by
Ram
39k • written 1 day ago by
beausoleilmo
▴ 530
0
votes
3
replies
188
views
Installation of PPFinder
gene-prediction
PPFinder
pseudogene
1 day ago by
subashini.fbtpb106
▴ 20
0
votes
4
replies
200
views
Difference between USCS exon coordinates and ensembl
exons
Ensembl
1 day ago by
amy__
▴ 50
3
votes
3
replies
244
views
Gene enrichment analysis
Go
enrichment
r
gene
updated 1 day ago by
GenoMax
129k • written 2 days ago by
Eliza
• 0
0
votes
0
replies
87
views
Why sone miRNAs are not present in TCGA data
TCGA
miRNA
RNA-seq
2 days ago by
nicolo.gualandi
• 0
0
votes
6
replies
305
views
Getting same value for start and end position, "DNA methylation"
DNAmethylation
methylkit
updated 1 day ago by
ATpoint
72k • written 2 days ago by
Pankaj
• 0
5
votes
6
replies
423
views
How to calculate TPM from featureCounts output
rna-seq
TPM
featurecounts
5 hours ago by
survive
• 0
3
votes
10
replies
1.7k
views
6 follow
How to split a scRNA reads BAM or FASTQ file to a separate file for each cell by cell barcode?
10xgenomics
RNAseq
scRNA
CB
updated 2 days ago by
biofalconch
▴ 580 • written 10 months ago by
MYousry
▴ 20
0
votes
0
replies
77
views
Prediction of altrered metabolites from altered gene expression
Gene
Metabolites
2 days ago by
english.server
▴ 290
0
votes
3
replies
173
views
CNNScoreVariants Error
CNNscore
updated 1 day ago by
Pierre Lindenbaum
154k • written 2 days ago by
bestone
▴ 10
2
votes
1
reply
129
views
Finding Enhancers using Genomation library
Genomation
updated 2 days ago by
ATpoint
72k • written 2 days ago by
Pankaj
• 0
0
votes
5
replies
436
views
convert data frame with character column to data frame with integer column
r
updated 2 days ago by
Basti
★ 1.6k • written 5 days ago by
atemedorri137798
• 0
0
votes
0
replies
144
views
Pore-C-Snakemake output files
contacts
updated 2 days ago by
zhang yi xing
▴ 10 • written 6 days ago by
oksana03fel
• 0
3
votes
3
replies
316
views
Why is coordinate sort required before findng read depths?
wgs
sambamba
updated 2 days ago by
zhang yi xing
▴ 10 • written 4 days ago by
guntul
• 0
2
votes
2
replies
162
views
how to sort unique seq from fasta files
fasta
updated 2 days ago by
shenwei356
8.1k • written 2 days ago by
Sapphire
• 0
2
votes
2
replies
204
views
I need help with a blast command, please help
linux
blast
updated 1 day ago by
Ram
39k • written 2 days ago by
neto.pvp
• 0
1
vote
1
reply
159
views
Is there a way to add a transcript to EnsDb.Hsapiens.v86?
ensembldb
GenomicFeatures
R
Bioconducter
EnsDb.Hsapiens.v86
2 days ago by
Daniel
▴ 30
2
votes
2
replies
158
views
How to make the profile line of `deepTools::plotHeatmap` more smooth?
plotHeatmap
deepTools
2 days ago by
Dan
▴ 120
0
votes
3
replies
225
views
Convert Bam file to Fastq
Fastq
Bam
updated 1 day ago by
Ram
39k • written 2 days ago by
وفاء
• 0
0
votes
0
replies
130
views
Job:
Data Engineer / Bioinformatician- AZ-Crick-Imperial College: Molecular Glue Discovery Partnership
data-engineering
drug-discovery
data-science
updated 1 day ago by
Ram
39k • written 2 days ago by
Sergio Martínez Cuesta
▴ 220
0
votes
6
replies
328
views
variant allelic fraction
vep
updated 20 hours ago by
Ram
39k • written 2 days ago by
Jom
• 0
0
votes
2
replies
167
views
WGCNA Trait File Issues
correlation
Deseq2
Gene
WGCNA
updated 2 days ago by
LChart
2.6k • written 2 days ago by
Jennifer M
• 0
0
votes
1
reply
145
views
Pairwise Alignment
Pairwise-Alignment
updated 1 day ago by
Ram
39k • written 2 days ago by
Fatemeh
• 0
3
votes
6
replies
2.0k
views
h5ad cellxgene to R
single-cell
h5
cellxgene
anndata
R
updated 2 days ago by
GenoMax
129k • written 6 months ago by
firestar
★ 1.5k
4
votes
6
replies
381
views
calculate p value and associated z score for snp-gene pair
snp
association
gene
pvalue
12 hours ago by
rheab1230
▴ 140
1
vote
3
replies
334
views
What is the benefit of knowing the source of contamination when we have RNAseq data with less than 70% mapping?
alignment
mapping
STAR
contamination
RNAseq
2 days ago by
Sib
▴ 40
0
votes
0
replies
95
views
How to perform a phylogenetic analysis from a vcf file
variants
haplotyping
phylogeny
vcf
2 days ago by
rj.rezwan
• 0
0
votes
1
reply
159
views
Where to get the molecular subtype information of STAD sample in TCGA
TCGA
database
updated 2 days ago by
Hamid Ghaedi
2.9k • written 3 days ago by
younglin113
▴ 50
0
votes
2
replies
230
views
Filtering mitochondrial reads from ATAC-Seq aligned reads- what to do with reads that have MT in RNEXT field
RNEXT
filtering
mitochondrial_reads
ATAC-Seq
samtools
updated 2 days ago by
benformatics
3.6k • written 3 days ago by
sam7459
• 0
1
vote
5
replies
374
views
Is a human genome file with reference as NCBI37 the same as hg19?
vcf
hg19
genome
reference-genome
updated 2 days ago by
GenoMax
129k • written 3 days ago by
a5864557
• 0
7
votes
4
replies
402
views
Forum:
[Meta] Use of AI
AI
Artificial-Intelligence
meta
ChatGPT
updated 1 day ago by
Ram
39k • written 3 days ago by
Vincent Laufer
★ 2.9k
0
votes
0
replies
136
views
How to keep ComplexHeatmap from reordering annotations
Dendrogram
HeatmapAnnotations
R
Heatmap
ComplexHeatmap
3 days ago by
Manuel
• 0
3
votes
5
replies
428
views
RNASeq gene labeling and mRNA filter from bulkRNA data.
RNA-Seq
RSubreads
updated 1 day ago by
rfran010
▴ 160 • written 5 days ago by
Yeeshouw
• 0
1
vote
2
replies
256
views
Random dataset and DESeq2
DESeq2
updated 3 days ago by
Vincent Laufer
★ 2.9k • written 3 days ago by
Saleh
• 0
1
vote
1
reply
220
views
Network cross-validation
wgcna
RNASeq
Network
CV
updated 3 days ago by
LChart
2.6k • written 4 days ago by
Arindam Ghosh
▴ 500
1
vote
2
replies
191
views
Differential protein expression analysis
R
proteomic
statistics
18 minutes ago by
Ribo
▴ 40
0
votes
3
replies
322
views
(ERR): "index/Trinity.fa" does not exist or is not a Bowtie 2 index Exiting now ...
bowtie2
align_stats
trinity
2 days ago by
eimanpharmacist
▴ 20
1
vote
2
replies
241
views
I have one fasta file of a bacteria. Can i use to create a phylogenetic tree?
Bioinformatics
WGS
3 days ago by
Mustafa
• 0
0
votes
4
replies
366
views
What are recommended parameters for the local protein alignment ?
protein
alignment
3 days ago by
Alexander
▴ 70
0
votes
0
replies
130
views
microsatellite, SSR, using Galaxy
Galaxy
SSR
using
microsatellite
4 days ago by
m.esmaeilpour
▴ 10
110,509 results • Page
2 of 2211
Recent Votes
A: Extract Reads From A Bam File That Fall Within A Given Region
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
Comment: Alignment of case vs. control from different origin
A: understanding bedtools coverage output
Answer: Alignment of case vs. control from different origin
Comment: Alignment of case vs. control from different origin
Comment: Gene prediction software
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Recent Replies
Comment: Differential protein expression analysis
by
Ribo
▴ 40
Thank you! Is there a recommended tutorial for proteomics analysis?
Comment: Limma returned only positive logFC values
by
melissachua90
▴ 40
I clustered the samples into subtypes and am now comparing the differential expression across the subtypes.
Answer: Limma returned only positive logFC values
by
Gordon Smyth
★ 6.1k
No, your code isn't correct. You are testing an intercept equal to zero instead of testing for a logFC. I wonder what comparison you were t…
Answer: Extract sequences from a fastq file by a list of IDs
by
GenoMax
129k
Using `filterbyname.sh` from [**BBMap suite**][1]: You need to include the `/1` in the header in your list file (here I am using the `nam…
Comment: Limma returned only positive logFC values
by
melissachua90
▴ 40
Yeah, I added the design matrix. `design <- model.matrix(~0+group)`. These are raw counts.
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
by
Ayish
• 0
Thank you for reply. I have Illumina paired-end reads. Would it be fine if I use hard-masking for STAR and soft-masked genome for BRAKER2? …
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
by
Darked89
4.4k
Soft masking the genome (unless something changed) not make any difference for STAR. Depending on how your RNA-Seq is done (Illumina? paire…
Answer: How to handle NaN in emmax Kinship matrix?
by
Thu
• 0
Hi! Thanks for sharing your solution! It works! :)
Comment: Gene prediction software
by
Darked89
4.4k
Looks like there are five Clarias genomes: * https://www.ncbi.nlm.nih.gov/genome/?term=txid13012[Organism:exp] The most complete seems t…
Comment: Alignment of case vs. control from different origin
by
sativus
▴ 10
Again, thank you so much for these very clearly explained summaries. After reading some articles on the matter, i feel i have a much better…
Answer: Extract sequences from a fastq file by a list of IDs
by
colindaven
4.9k
Some people I know have used filter-fastq successfully: https://github.com/Floor-Lab/filter-fastq
Comment: Extract sequences from a fastq file by a list of IDs
by
Pierre Lindenbaum
154k
> but none of them work for me https://meta.stackexchange.com/questions/147616/
Comment: Extract sequences from a fastq file by a list of IDs
by
Pierre Lindenbaum
154k
https://meta.stackexchange.com/questions/147616/
Comment: Extract sequences from a fastq file by a list of IDs
by
mhpakdel96
• 0
I have tried this one before but the output file is empty
Comment: Extract sequences from a fastq file by a list of IDs
by
mhpakdel96
• 0
I have tried all of them but none of them work for me
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