Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
116,611 results • Page
2 of 2333
Sort: replies
Rank
Views
Votes
Replies
32
votes
51
replies
20k
views
8 follow
Getting Unmapped Reads: Comparing Fastq To Bam
fastq
samtools
bedtools
bam
updated 12.4 years ago by
brentp
24k • written 12.4 years ago by
User 9996
▴ 840
27
votes
50
replies
18k
views
7 follow
Extract fasta sequences from a large file using a list of names
fasta
updated 14 months ago by
Ram
43k • written 9.0 years ago by
fhsantanna
▴ 610
22
votes
50
replies
17k
views
Why I can not install anything on R?
R
software error
5.5 years ago by
Za
▴ 140
93
votes
50
replies
16k
views
17 follow
Forum:
Fda Sends A Warning Letter To 23Andme - Personal Genomics Service Marketing To Be Discontinued
personal-genomics
23andMe
updated 14 months ago by
Ram
43k • written 10.4 years ago by
Istvan Albert
100k
26
votes
50
replies
14k
views
8 follow
Tool:
Retrieve a subset of FASTA from large Illumina multi-FASTA file
fasta
multi-fasta
illumina
updated 10 months ago by
Ram
43k • written 7.9 years ago by
hcwang
▴ 50
12
votes
50
replies
4.1k
views
How can I find out the Linux version and what is the ftp command to install R and R Studio on Linux server?
R
7.1 years ago by
thomas.f.hahn2
▴ 100
188
votes
50
replies
73k
views
41 follow
How To Remove The Same Sequences In The Fasta Files?
fasta
sequence
duplicates
updated 19 months ago by
Ram
43k • written 13.5 years ago by
Zhangleisdau
▴ 340
92
votes
50
replies
53k
views
16 follow
PCA plot from read count matrix from RNA-Seq
RNA-Seq
pca
updated 2.4 years ago by
BioNovice247
▴ 20 • written 6.5 years ago by
rachel.kubik12
▴ 220
85
votes
49
replies
10k
views
31 follow
Which Application Is Truly Missing In Bioinformatics?
subjective
general
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Jarretinha
3.4k
262
votes
48
replies
23k
views
37 follow
Forum:
Bioinformatics courses, workshops or training
Workshop
Training
Courses
updated 14 months ago by
Lars
★ 1.0k • written 9.0 years ago by
Deepak Tanwar
★ 4.2k
39
votes
48
replies
7.0k
views
CUTADAPT in windows
sequencing
updated 14 months ago by
Ram
43k • written 9.0 years ago by
zizigolu
★ 4.3k
90
votes
48
replies
33k
views
29 follow
Tutorial:
Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm
rna-seq
bioconductor
updated 5 months ago by
Ram
43k • written 10.5 years ago by
Irsan
★ 7.8k
72
votes
48
replies
18k
views
15 follow
Tutorial:
For Short Reads, Which Aligners Find All Hits Given Certain Edit Distance Threshold?
ngs
alignment
bwa
updated 14 months ago by
Ram
43k • written 11.2 years ago by
lh3
33k
131
votes
48
replies
16k
views
19 follow
Justifying Learning Linux For Bioinformatics
linux
subjective
updated 12.7 years ago by
Guangchuang Yu
★ 2.6k • written 12.7 years ago by
Eric Normandeau
11k
68
votes
48
replies
8.7k
views
16 follow
Forum:
Stranger Things: unexpected limitations of popular tools
samtools
hisat2
updated 11 months ago by
Ram
43k • written 6.8 years ago by
Istvan Albert
100k
72
votes
48
replies
8.5k
views
18 follow
What Kind Of Bioinformatics Tutorials Would You Like To See Online?
education
updated 14 months ago by
Ram
43k • written 13.0 years ago by
User 59
13k
22
votes
47
replies
5.3k
views
(solved) I couldn't reproduce the problem of max_target_seqs
blast
updated 5.1 years ago by
gb
★ 2.2k • written 5.6 years ago by
fishgolden
▴ 510
23
votes
47
replies
7.1k
views
6 follow
Dbsnp Individual Genotyping Information For Specific Snps
dbsnp
updated 13.1 years ago by
Khader Shameer
18k • written 13.1 years ago by
Andrea_Bio
★ 2.8k
18
votes
47
replies
4.7k
views
Fastq Trimmer by pattern
fastq
trimming
reads
terminal
7.2 years ago by
dzisis1986
▴ 70
188
votes
47
replies
2.8k
views
16 follow
Job:
Bioinformatics freelancers needed
freelancer
updated 12 months ago by
Ram
43k • written 8.0 years ago by
Bioinfexperts
▴ 190
176
votes
46
replies
19k
views
26 follow
Forum:
Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by
Istvan Albert
100k
43
votes
46
replies
4.2k
views
taking sequences of a list of miRNAs
miRNA
mirbase
sequence
gene
updated 7.5 years ago by
Ram
43k • written 7.5 years ago by
zizigolu
★ 4.3k
105
votes
46
replies
13k
views
13 follow
Forum:
Why Does Biostar Cover Questions On Epigenetics, But Not Intelligent Design?
epigenetics
updated 14 months ago by
Ram
43k • written 10.7 years ago by
ugly.betty77
★ 1.1k
126
votes
46
replies
50k
views
21 follow
Forum:
Visualization of ChIP-seq data using Heatmaps (Updated: 06/10/16)
visualization
ChIP-Seq
homer
ngsplot
deeptools
updated 12 months ago by
Ram
43k • written 8.1 years ago by
Sinji
★ 3.2k
83
votes
46
replies
8.8k
views
15 follow
Tool:
ChatGPT optimized for bioinformatics questions
gpt
updated 6 weeks ago by
LauferVA
4.2k • written 10 months ago by
Sasha
▴ 840
37
votes
46
replies
6.8k
views
12 follow
Quick Programming Challenge: How Do I Calculate Reference Coverage From A Table Of Alignment Starts And Ends?
code
alignment
programming
updated 5.6 years ago by
Ram
43k • written 14.0 years ago by
Jeremy Leipzig
22k
92
votes
46
replies
12k
views
28 follow
Best Language For Introductory Programming Course From Within An Introduction Course On Bioinformatics.
programming
subjective
updated 14 months ago by
Ram
43k • written 12.8 years ago by
Andra Waagmeester
3.2k
237
votes
45
replies
72k
views
34 follow
What Is The Best Pipeline For Human Whole Exome Sequencing?
next-gen
sequencing
illumina
exome
human
updated 12.9 years ago by
User 9126
▴ 50 • written 13.9 years ago by
Biomed
5.0k
108
votes
45
replies
86k
views
30 follow
How To Extract A Sequence From A Big (6Gb) Multifasta File ?
fasta
updated 20 months ago by
aswinssoman
▴ 70 • written 11.8 years ago by
Mchimich
▴ 320
4
votes
45
replies
7.7k
views
SAM file wrong? help with validatesamfile
EXOME
updated 12 months ago by
Ram
43k • written 7.8 years ago by
cristina_sabiers
▴ 110
185
votes
45
replies
32k
views
27 follow
How To Organize A Pipeline Of Small Scripts Together?
pipeline
general
updated 6 months ago by
Ram
43k • written 14.2 years ago by
Giovanni M Dall'Olio
28k
22
votes
45
replies
13k
views
9 follow
Tool to separate human and mouse rna seq reads
RNA-Seq
next-gen-sequencing
updated 15 months ago by
Ram
43k • written 8.9 years ago by
Ron
★ 1.2k
46
votes
44
replies
25k
views
9 follow
bash loop for alignment RNA-seq data
RNA-Seq
next-gen
alignment
updated 19 months ago by
Ram
43k • written 10.0 years ago by
Paul
★ 1.5k
130
votes
44
replies
34k
views
16 follow
Forum:
Trimming adapter sequences - is it necessary?
NGS
adapter
RNA-Seq
updated 13 months ago by
Ram
43k • written 7.6 years ago by
Lars
★ 1.0k
43
votes
44
replies
5.7k
views
14 follow
Macbook M1 pro/M2 pro or Dell (with Ubuntu/other linux distribution) for bioinformatics stuff?
metagenomics
amplicon
macbook
Intel
MacOs
updated 6 months ago by
steve
★ 3.5k • written 6 months ago by
l.gallucci
▴ 10
90
votes
44
replies
60k
views
28 follow
Tool to generate proportional Venn Diagrams?
visualization
updated 2.0 years ago by
Ram
43k • written 13.0 years ago by
Ryan D
★ 3.4k
121
votes
44
replies
21k
views
21 follow
What Is Your Favorite Question To Ask When Interviewing Potential Bioinformaticians?
subjective
updated 14 months ago by
Ram
43k • written 13.9 years ago by
Madelaine Gogol
5.3k
27
votes
44
replies
15k
views
How to remove contamination from the transcriptome assembly
RNA-Seq
contamination
assembly
blast
Hiseq reads
updated 20 months ago by
Ram
43k • written 8.5 years ago by
seta
★ 1.9k
196
votes
44
replies
72k
views
33 follow
Tutorial:
Analysing Microarray Data In Bioconductor
microarray-analysis
bioconductor
updated 15 months ago by
Ram
43k • written 11.6 years ago by
Obi Griffith
20k
155
votes
44
replies
32k
views
29 follow
Which Bioinformatic Friendly Pipeline Building Framework?
scripting
updated 22 months ago by
Ram
43k • written 10.3 years ago by
Carlos Borroto
★ 2.1k
99
votes
44
replies
24k
views
33 follow
Best Free Text Editor For All Popular Languages (R, Python, Perl Etc..)
text
r
perl
python
updated 6.6 years ago by
grant.hovhannisyan
★ 2.6k • written 12.1 years ago by
John
★ 1.5k
21
votes
44
replies
4.3k
views
How to analyze Illumina RNASeq data
RNA-Seq
sequencing
next-gen
3.7 years ago by
Kumar
▴ 170
185
votes
44
replies
83k
views
30 follow
Drawing Chromosome Ideograms With Data
ideogram
visualization
chromosome
updated 5 weeks ago by
Michael
54k • written 14.1 years ago by
Yuri
★ 1.7k
102
votes
44
replies
9.1k
views
22 follow
What Are Numbers Every Bioinformatician Should Know?
knowledge
career
updated 14 months ago by
Ram
43k • written 10.7 years ago by
brentp
24k
122
votes
44
replies
11k
views
24 follow
News:
Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta
biostars
updated 6 months ago by
Ram
43k • written 11.4 years ago by
Istvan Albert
100k
1
vote
43
replies
4.0k
views
Database from .gbk files
database
gbk
ncbi
updated 12 months ago by
Raul
• 0 • written 3.9 years ago by
graysonford
▴ 10
16
votes
43
replies
6.7k
views
Stuck on calculating principal components
Vegan
R
software error
SNP
updated 6.4 years ago by
pfs
▴ 280 • written 6.4 years ago by
zizigolu
★ 4.3k
66
votes
43
replies
27k
views
23 follow
Ngs - Huge (Fastq) File Parsing - Which Language For Good Efficiency ?
next-gen
sequencing
parsing
fastq
programming
updated 2.2 years ago by
Ram
43k • written 13.3 years ago by
toni
★ 2.2k
31
votes
43
replies
19k
views
11 follow
Volcano plot help code
R
rna-seq
updated 5.8 years ago by
jordi.planells
▴ 480 • written 6.0 years ago by
anasofiamoreira94
▴ 80
59
votes
43
replies
21k
views
18 follow
Tutorial:
Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign
pacbio
arrow
smrt-limk
updated 13 months ago by
Ram
43k • written 6.6 years ago by
Rox
★ 1.4k
116,611 results • Page
2 of 2333
Recent Votes
Comment: Sequence read length shorter than flow cell specification
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
Detecting somatic variants in non-tumor tissue without normals
Answer: Sequence read length shorter than flow cell specification
Answer: Sequence read length shorter than flow cell specification
Comment: Why gatk VariantAnnotator required bam and coverage files
A: Generate Vcf.Gz File And Its Index File Vcf.Gz.Tbi
Recent Locations •
All
Whitefish, MT,
just now
Turkey,
2 minutes ago
Cambridge, MA,
8 minutes ago
United States,
9 minutes ago
Switzerland,
10 minutes ago
Canada,
10 minutes ago
United States,
10 minutes ago
Recent Awards •
All
Scholar
to
ATpoint
82k
Popular Question
to
zdiazmar
▴ 30
Popular Question
to
hkarakurt
▴ 180
Student
to
John Torcivia
▴ 70
Popular Question
to
bioinfo
▴ 150
Popular Question
to
jvijai
★ 1.2k
Scholar
to
otieno43
▴ 30
Recent Replies
Answer: Rare Disease Variant Pathway Analysis
by
LauferVA
4.2k
Hi @efc1e545 , First a caveat. the information we most need in order to help guide you to a successful conclusion is not provided in thi…
Answer: Sequence read length shorter than flow cell specification
by
swbarnes2
14k
The company probably had you share your run with someone who needed the extra bases. So you get the extra bases free. Just use them unle…
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
Ram
43k
Thank you, the `csvtk spread` is super useful. I usually import into R using `lapply` then `Reduce` using `merge` but this might be easier.
Comment: Annovar using R package
by
DKA
▴ 40
Thank you for your guidance. The thing is that I am unfamiliar with using such environments, unfortunately.
Comment: Treatment VS Control in Single Cell RNAseq analysis
by
ATpoint
82k
Open a new question, with details.
Comment: Normalize scRNAseq data to housekeeping genes to compare several datasets
by
ATpoint
82k
I recommend https://bioconductor.org/books/3.18/OSCA.basic/normalization.html as well as the "advanced" section in this book.
Comment: Why gatk VariantAnnotator required bam and coverage files
by
QX
• 0
thank you!
Comment: Treatment VS Control in Single Cell RNAseq analysis
by
kilcdincer
▴ 10
Hello, I have more or less same experimental setting and was wondering how you proceeded with your analysis? Can I reach its GitHub reposit…
Comment: BLAST using both nucleotides and taxonomic local databases
by
GenoMax
141k
> I ask this as some BLAST command fields as scinames or sblastnames do not give any output with a classic nt BLAST If that information is…
Answer: BLAST using both nucleotides and taxonomic local databases
by
5heikki
11k
This has been discussed [many times][1] [1]: https://www.biostars.org/p/76551/
Answer: why renaming Idents in Seurat object doesn't work?
by
Bioinfotec
▴ 10
I think when you give indent to seuObj : ```r Idents(seuObj) <- 'RNA_snn_res.0.1' ``` You may wrongly assign other value to it such as `…
Answer: ScRNAseq-How to correctly choose cell type marker genes
by
Bioinfotec
▴ 10
In fact, there is no gold standard for cell type annotation. To achieve correct cell type classification, you can combine the two methos. F…
Answer: ScRNA data question
by
Bioinfotec
▴ 10
In violin plot, a dot represents a cell. Actually, the straight lines you see in your infected image just means these cells have the same e…
Comment: Sequence read length shorter than flow cell specification
by
GenoMax
141k
> the have sent data with different sequencing read lengths: 55bp pair ended and 80bp single ended If they sent data that is not what you…
Comment: How to know synonymous and non-synonymous SNPs from transcriptome sequencing
by
rohitsatyam102
▴ 850
I have some off-the-chart dN/dS values for few genes (>20) when using your script on VCF obtained from a 20K plasmodium genomes (pf7K). | …
Traffic: 2567 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6