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831 results • Page
2 of 17
Sort: replies
Rank
Views
Votes
Replies
16
votes
10
replies
10k
views
8 follow
GRCh37.p13 download at NCBI FTP?
ncbi
reference
grch37
updated 19 days ago by
Sruthi
• 0 • written 5.5 years ago by
Marvin
▴ 220
3
votes
10
replies
573
views
Errors running PHG MakeInitialPHGDBPipelinePlugin (**UNASSIGNED**)
pangenome
plants
PHG
updated 15 days ago by
lcj34
▴ 410 • written 17 days ago by
b.contreras.moreira
▴ 70
18
votes
10
replies
5.2k
views
Should I normalize read counts if I want to run plotPCA?
RNA-Seq
updated 20 days ago by
ella
• 0 • written 3.9 years ago by
c_u
▴ 520
16
votes
10
replies
655
views
6 follow
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 25 minutes ago by
Madelaine Gogol
5.3k • written 22 hours ago by
Ann
★ 2.4k
6
votes
10
replies
2.7k
views
How can Investigate if a Viral genome is circular or linear? And if it is single or double stranded?
Assembly
genome
updated 27 days ago by
Israel
• 0 • written 5.3 years ago by
GiV17
▴ 50
3
votes
10
replies
657
views
Intersect gene IDs list and GFF3 to get the annotation.
annotation
GFF
6 days ago by
Lissa Cruz Saavedra
• 0
0
votes
10
replies
781
views
Problem running scPred
scPred
scPredict
updated 15 days ago by
Ram
43k • written 12 weeks ago by
Nitin
• 0
2
votes
10
replies
730
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 23 hours ago by
Ram
43k • written 5 weeks ago by
Sky
▴ 10
3
votes
10
replies
705
views
t-test in two groups, multiple rows
R
t-test
10 days ago by
sooni
▴ 20
15
votes
10
replies
7.5k
views
6 follow
Intersect multiple BED files
bed
intersect
updated 1 day ago by
Alex Reynolds
35k • written 8.2 years ago by
int11ap1
▴ 470
2
votes
10
replies
562
views
Can't properly use GATK Mutect2 to call somatic variants from my tumor/normal samples pair (BAM to VCF)
vaf
gatk
vcf
genomics
mutect2
updated 16 days ago by
GenoMax
140k • written 16 days ago by
Samuel
▴ 20
4
votes
10
replies
620
views
PDB residue serial inconsistency and PDB id&UniProt ID mismatch
pdb
UniProtID
pdbID
updated 28 days ago by
Wayne
★ 1.9k • written 29 days ago by
Nafi
• 0
5
votes
9
replies
3.7k
views
Can one plot % of variance using the plotMDS function?
limma
plotMDS
PCA
PCoA
% of varience
updated 27 days ago by
Gordon Smyth
★ 6.9k • written 3.8 years ago by
harelarik
▴ 90
1
vote
9
replies
3.5k
views
Error in CIBERSORTx
RNA-seq
CIBERSORT
LM22
updated 19 hours ago by
Ram
43k • written 17 months ago by
Manav
• 0
0
votes
9
replies
487
views
RNA_seq
RNA-seq
updated 14 days ago by
swbarnes2
14k • written 15 days ago by
daffodil
▴ 10
4
votes
9
replies
504
views
Max length reads from fastq
fastq
updated 23 days ago by
Michael
54k • written 24 days ago by
marco.barr
▴ 30
1
vote
9
replies
441
views
miRNA alignment with Bowtie2
bowtie2
13 days ago by
Chironex
▴ 40
1
vote
9
replies
802
views
Python3 or Ubuntu, not perl: Have Primers with Degenerate Bases, Need tool or way to List all Possible Nucleotide Sequences
degenerate-bases
python
primers
biopython
updated 17 days ago by
Dave Carlson
★ 1.7k • written 17 days ago by
Matthew
• 0
4
votes
9
replies
935
views
Error in openning FastQC
fastqc
igv
updated 15 hours ago by
GenoMax
140k • written 7 months ago by
huynhthienphai
• 0
0
votes
9
replies
304
views
Low number of both surviving reads after trimming
trimmomatic
trimming
15 hours ago by
Jay
• 0
6
votes
9
replies
1.8k
views
How to convert .wib files to .wig or .bed?
wib
BED
wig
bedtools
2 days ago by
Zeng Jingyu
▴ 60
14
votes
9
replies
11k
views
8 follow
Downsample BAM file to specific amount of reads
bam
updated 14 days ago by
GenoMax
140k • written 2.6 years ago by
kstangline
▴ 80
0
votes
9
replies
462
views
Nextflow ERROR : Timeout waiting for connection from pool
nextflow
updated 1 day ago by
GenoMax
140k • written 7 days ago by
dzisis1986
▴ 70
5
votes
9
replies
631
views
Porechop output?
porechop
Nanopore
updated 28 days ago by
GenoMax
140k • written 4 weeks ago by
giulia.trauzzi
▴ 10
0
votes
9
replies
453
views
Intersect genomic locations with genes
fanc
Chromatin
conformation
Hic
hic
updated 7 days ago by
zx8754
11k • written 7 days ago by
naveedhasan2000
• 0
4
votes
9
replies
1.5k
views
Cutadapt error: too many parameters.
cutadapt
parallel
bash
updated 23 days ago by
ATpoint
81k • written 10 months ago by
DanielEB_fisk
▴ 20
4
votes
9
replies
671
views
Snakemake >= version 8 cluster configuration and SLURM job submission
parallelization
slurm
snakemake
cluster
updated 3 days ago by
Simon
▴ 10 • written 7 days ago by
Maxwell
▴ 20
1
vote
9
replies
1.4k
views
blast retrieves "N/A" for taxonomic data
blast
blastn
updated 19 days ago by
flavianegrete
• 0 • written 3.3 years ago by
langziv
▴ 50
0
votes
8
replies
420
views
STAR aligner options
STAR
1 day ago by
theophile
• 0
7
votes
8
replies
540
views
Forum:
Bioinformatics career transition
career
forum
updated 14 days ago by
jared.andrews07
★ 16k • written 14 days ago by
jevill17
▴ 20
9
votes
8
replies
5.6k
views
7 follow
CONTRA for CNV detection. troubleshooting
genome
next-gen-sequencing
cnv
software-error
updated 22 hours ago by
Ram
43k • written 9.8 years ago by
Kizuna
▴ 870
3
votes
8
replies
6.2k
views
6 follow
Download CAZy database
genome
gene
database
cazy
updated 14 days ago by
GenoMax
140k • written 6.1 years ago by
anu014
▴ 190
7
votes
8
replies
412
views
Create Conda Environment with YAML File
Python
updated 14 days ago by
dthorbur
★ 1.7k • written 15 days ago by
cthangav
▴ 100
2
votes
8
replies
266
views
Piping samtools to R
R
NGS
bash
samtools
1 day ago by
joe
▴ 470
5
votes
8
replies
2.2k
views
VariantCalling with single sanger read
snps
blast
indel
Variant-Calling
updated 3 days ago by
Ram
43k • written 7.6 years ago by
Amirosein
▴ 70
4
votes
8
replies
470
views
i'm trying to use STAR and there is a problem...
STAR
updated 19 days ago by
GenoMax
140k • written 19 days ago by
markusz
▴ 10
1
vote
8
replies
684
views
6 follow
Using aspera-connect to UPLOAD fastq files to ENA on Linux
ENA
aspera-connect
ascp
updated 14 days ago by
jude
• 0 • written 15 days ago by
sue.jones
• 0
1
vote
8
replies
3.2k
views
cutadapt error problem
next-gen
cutadapt
updated 21 hours ago by
Ram
43k • written 5.5 years ago by
amitpande74
▴ 20
0
votes
8
replies
1.4k
views
Cannot find Output files after applying Markduplicates with picard tools
picard-tools
MarkDuplicates
updated 14 days ago by
xiangbinruan
• 0 • written 18 months ago by
Phoebe Magdy
• 0
17
votes
8
replies
5.0k
views
Mean Variance Relationship single cell RNA-Seq Data
RNA-Seq
variance
deseq2
single cell
updated 17 days ago by
thomas.heigl.ibk
• 0 • written 3.3 years ago by
kw486
▴ 30
11
votes
8
replies
5.0k
views
Multi-mapping High with featureCounts but not STAR
RNA-Seq
sequencing
alignment
updated 8 days ago by
GenoMax
140k • written 4.8 years ago by
garbuzov
▴ 70
1
vote
8
replies
342
views
0-based coordinates from samtools depth
samtools
coverage
depth
updated 4 days ago by
Pierre Lindenbaum
160k • written 4 days ago by
marco.barr
▴ 30
2
votes
8
replies
441
views
Expert Guidance Required for De Novo Transcriptome Analysis
Transcriptome
De-novo
updated 8 days ago by
GenoMax
140k • written 11 days ago by
ben@f
▴ 10
2
votes
8
replies
1.2k
views
How can I linearize certain residues within an existing protein structure to create a dumbbell-shaped structure?
pymol
python
biopython
structural-bioinformatics
updated 23 days ago by
Wayne
★ 1.9k • written 5 weeks ago by
Aman Vishwakarma
• 0
5
votes
8
replies
516
views
Difference between GLM and Wilcoxon rank sum test
GLM
rank-sum
Wilcoxon
22 days ago by
yxwucq
• 0
0
votes
8
replies
510
views
Fine-tuning NCBI taxid / taxidlist
BLAST
taxonomy
7 days ago by
theclubstyle
▴ 40
12
votes
7
replies
6.7k
views
Forum:
Differences between SMART-seq2, SMART-seq3, and 10x
cell
smartseq
UMIs
single
10x
updated 4 days ago by
dsull
★ 5.7k • written 2.6 years ago by
hamarillo
▴ 70
3
votes
7
replies
513
views
wANNOVAR memory error
VCF
Annovar
updated 22 days ago by
vinayjrao
▴ 250 • written 7 weeks ago by
colourful_frog
• 0
0
votes
7
replies
1.1k
views
Pan genome of genes
Get_homologues
updated 17 days ago by
b.contreras.moreira
▴ 70 • written 2.9 years ago by
Pedro
• 0
3
votes
7
replies
3.4k
views
WGCNA adjust p value
statistics
WGCNA
updated 14 days ago by
Ceren
• 0 • written 2.7 years ago by
jason.taotaotan
▴ 10
831 results • Page
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Recent Votes
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Bulk RNAseq from any sample is still considered a bulk sample. Running bulk RNA sequencing on a sample with any level of cell type/state c…
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This is going to be tough to diagnose. Are you going to align to a reference? If so go ahead with the alignments to see if alignments turn …
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Thank you for replying. I have separated the reads with at least one N on them and they account for 0.2% of the total reads in the file (8'…
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