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799 results • Page
1 of 16
Sort: Votes
Rank
Views
Votes
Replies
298
votes
122
replies
28k
views
29 follow
Mean Length Of Fasta Sequences
code
fasta
sequence
codegolf
updated 19 days ago by
jena
▴ 270 • written 13.2 years ago by
Eric Normandeau
11k
170
votes
41
replies
91k
views
27 follow
Multiline Fasta To Single Line Fasta
fasta
updated 22 hours ago by
Ram
40k • written 12.3 years ago by
Palu
▴ 250
162
votes
64
replies
80k
views
39 follow
Selecting Random Pairs From Fastq?
random
fastq
illumina
sequence
updated 4 days ago by
rohitsatyam102
▴ 750 • written 12.5 years ago by
Ketil
4.1k
146
votes
70
replies
46k
views
20 follow
Tutorial:
Network plot from expression data in R using igraph
microarray
RNA-Seq
R
network
updated 26 days ago by
Raheleh
▴ 260 • written 5.8 years ago by
Kevin Blighe
86k
124
votes
13
replies
165k
views
13 follow
How Can I Convert Bam To Sam?
bam
next-gen-sequencing
sam
updated 18 days ago by
Ram
40k • written 13.2 years ago by
Biomed
4.9k
105
votes
26
replies
40k
views
23 follow
How To Create Mutation Diagram In R Or In Any Tools?
mutation
domain
r
updated 5 days ago by
zx8754
11k • written 10.7 years ago by
henryvuong
▴ 810
100
votes
38
replies
28k
views
34 follow
Forum:
List of cloud genomics companies
cloud-genomics
updated 24 days ago by
Jeremy Leipzig
21k • written 9.9 years ago by
14134125465346445
★ 3.6k
81
votes
21
replies
40k
views
15 follow
How To Get Bed File Containing Exons Of Canonical Transcripts And Their Corresponding Gene Symbols
bed
transcript
ucsc
biomart
updated 9 days ago by
Joel Wallenius
▴ 180 • written 9.6 years ago by
pristanna
▴ 750
75
votes
18
replies
2.0k
views
11 follow
Blog:
A list of technical advantages of Snakemake over Nextflow | Cloud solution for Snakemake
Snakemake
updated 4 days ago by
Ram
40k • written 9 days ago by
Kenny
▴ 30
60
votes
21
replies
2.8k
views
14 follow
Reproduce the article "The complete sequence of a human genome."
genome
assembly
updated 22 days ago by
Michael
53k • written 23 days ago by
sqshigg
▴ 60
38
votes
25
replies
8.1k
views
13 follow
FASTA file of fixed length
sequence
fasta
updated 10 days ago by
Ram
40k • written 7.5 years ago by
waqasnayab
▴ 250
36
votes
5
replies
5.8k
views
Tutorial:
[Beginner] Introduction to bioinformatics file types
fastq
fasta
bed
SAM
updated 15 days ago by
gallardodiazmiriam
▴ 20 • written 8.2 years ago by
Luke Crichton
▴ 410
36
votes
16
replies
28k
views
14 follow
Generating consensus sequence from bam file
genome
samtools
bwa
fasta
updated 3 days ago by
jkbonfield
★ 1.1k • written 4.6 years ago by
chparada
▴ 60
35
votes
23
replies
27k
views
11 follow
bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open shared object file: No such file or directory
bcftools
updated 21 days ago by
s.w.vanderlaan
▴ 40 • written 2.2 years ago by
Michal Nevo
▴ 110
33
votes
23
replies
18k
views
12 follow
Is there a tool that sorts gtf files?
RNA-Seq
genome
updated 10 days ago by
alejandrogzi
▴ 30 • written 5.5 years ago by
JJ
▴ 660
23
votes
12
replies
1.9k
views
Genes encoded on both strands of DNA?
Genes
strand
updated 2 days ago by
Ram
40k • written 16 months ago by
A_heath
▴ 140
21
votes
9
replies
21k
views
9 follow
Building Snpeff Database
vcftools
snp
gatk
updated 18 days ago by
Ram
40k • written 11.1 years ago by
bioinfo
▴ 830
21
votes
8
replies
30k
views
8 follow
Merging/Concatenating Vcf Files
vcftools
snp
indel
updated 29 days ago by
Bioinformatics_NewComer
▴ 330 • written 11.1 years ago by
bioinfo
▴ 830
19
votes
10
replies
825
views
Forum:
Pain points using commercial clouds
gpt
tinybio
updated 8 days ago by
vincenthus
▴ 70 • written 23 days ago by
Sasha
▴ 720
19
votes
10
replies
29k
views
6 follow
read depth using samtools
depth
samtools
updated 9 days ago by
Sachin
• 0 • written 4.7 years ago by
LimMo
▴ 30
19
votes
14
replies
793
views
Forum:
What is the amount of sequencing data produced annually?
data
research
sequencing
updated 2 days ago by
Jeremy Leipzig
21k • written 3 days ago by
vincenthus
▴ 70
18
votes
14
replies
26k
views
7 follow
removing batch effects using ComBat and SVA
R
ComBat
SVA
Batch effect
updated 29 days ago by
maximal_life
▴ 20 • written 7.3 years ago by
LJ
▴ 260
18
votes
10
replies
14k
views
Could you please explain Fold change, % of change, and log2 fold change (L2FC) to a layment?
gene
RNA-Seq
next-gen
fold-change
updated 8 days ago by
Ram
40k • written 3.9 years ago by
WUSCHEL
▴ 720
18
votes
22
replies
8.0k
views
10 follow
Tutorial:
Create de novo repeat library
de-novo
repeat
annotation
updated 23 hours ago by
evoecogen
▴ 20 • written 3.8 years ago by
Juke34
8.2k
16
votes
16
replies
1.8k
views
Species identifcation using 16s rRNA
16srRNA
fasta
updated 2 days ago by
Ram
40k • written 2.6 years ago by
A_heath
▴ 140
13
votes
5
replies
948
views
Annotating TSS: By Transcript or by Gene? Code Validation Help Needed!
genome
bed
transcript
TSS
updated 7 days ago by
i.sudbery
17k • written 4 months ago by
Rafael Soler
★ 1.2k
13
votes
13
replies
6.5k
views
6 follow
Extract SNPs flanking sequences based on VCF and genome Fasta files
snp
sequence
genome
updated 25 days ago by
LauGuillardin
• 0 • written 5.1 years ago by
Denis
▴ 280
12
votes
5
replies
13k
views
How to combine chromosome vcf files
Assembly
alignment
next-gen
genome
updated 22 days ago by
Pierre Lindenbaum
157k • written 5.3 years ago by
williamsbrian5064
▴ 480
12
votes
16
replies
5.2k
views
8 follow
ACMG assignment tools
wes
acmg
WES
WGS
updated 29 days ago by
zasnuty
• 0 • written 3.3 years ago by
Eugene A
▴ 170
11
votes
8
replies
749
views
6 follow
Forum:
How to write a review article in bioinformatics
article
review
updated 22 days ago by
Ram
40k • written 6 weeks ago by
joey
▴ 10
11
votes
4
replies
903
views
How to determine % similarity between genomes?
mauve
mugsy
genome-alignment
updated 2 days ago by
Ram
40k • written 2.5 years ago by
A_heath
▴ 140
11
votes
7
replies
3.4k
views
7 follow
Assembler for only nanopore data
genomics
Assembly
ONT
Abyss
updated 12 days ago by
GenoMax
134k • written 4.4 years ago by
ringourquia
▴ 20
10
votes
11
replies
1.2k
views
Seeking feedback on ChIP-seq normalization method: Calculating scaling factors by dividing input by IP, including spike-in
coverage
spike-in
scaling-factor
ChIP-seq
14 days ago by
kalavattam
▴ 180
10
votes
9
replies
1.1k
views
Help interpretating DESeq2 output
Deseq2
Fold-Change
RNA-seq
updated 8 days ago by
Ram
40k • written 2.8 years ago by
nanoide
▴ 100
10
votes
5
replies
25k
views
FastQC with multiple FASTQ files
ngs
fastqc
multiple
updated 4 days ago by
Ram
40k • written 5.2 years ago by
m98
▴ 400
10
votes
8
replies
459
views
Adding a control sample to bulk RNA-seq
RNA-seq
1 day ago by
Chris
▴ 180
10
votes
8
replies
11k
views
8 follow
How to install gdc-client in Ubnutu
ubuntu
gdc-client
updated 12 days ago by
Ram
40k • written 7.1 years ago by
pxf109
▴ 20
10
votes
13
replies
12k
views
6 follow
How to annotate only selected genes on a heatmap
R
updated 18 days ago by
GenoMax
134k • written 6.3 years ago by
h.fushimi.x689
▴ 30
10
votes
18
replies
3.1k
views
how to identify CDR region in antibody sequence
CDR
antibody
updated 22 days ago by
Ram
40k • written 15 months ago by
reany
▴ 50
9
votes
3
replies
390
views
Seeking a real project
thesis
project
updated 10 days ago by
Ram
40k • written 11 days ago by
m.ranjbar2009
▴ 20
9
votes
8
replies
5.2k
views
7 follow
CONTRA for CNV detection. troubleshooting
genome
next-gen
cnv
software-error
sequencing
updated 21 days ago by
Avinash
• 0 • written 9.3 years ago by
Kizuna
▴ 860
9
votes
6
replies
1.2k
views
6 follow
Forum:
GTF files from Ensembl Releases 105 and 106 unsorted
Ensembl
bug
GTF
updated 10 days ago by
ATpoint
76k • written 10 months ago by
dlaehnemann
▴ 30
9
votes
8
replies
3.4k
views
bcftools multiallelic split not working
bcftools
vcf
updated 21 days ago by
DareDevil
★ 3.3k • written 2.1 years ago by
from the mountains
▴ 220
9
votes
5
replies
7.0k
views
Genomic Bam To Transcriptome Bam
bam
samtools
updated 4 days ago by
Ram
40k • written 9.8 years ago by
dfernan
▴ 750
9
votes
7
replies
6.7k
views
Gc Content From Bam
gc
bam
awk
perl
updated 21 days ago by
Ram
40k • written 9.6 years ago by
filipzembol
▴ 170
9
votes
4
replies
1.1k
views
Illumina Q score
Illumina
quality-score
updated 2 days ago by
Ram
40k • written 2.0 years ago by
A_heath
▴ 140
9
votes
4
replies
1.2k
views
How to extend contigs from single-end reads?
contigs
contig-extension
updated 2 days ago by
Ram
40k • written 3.0 years ago by
A_heath
▴ 140
9
votes
5
replies
4.2k
views
CWL: how to set default values
Common-Workflow-Language
cwl
updated 11 days ago by
Ram
40k • written 6.9 years ago by
bird77
▴ 80
9
votes
12
replies
4.2k
views
converting a list of genes and interaction to a graph
gene
R
updated 2 days ago by
Amirhossein Hajianpour
▴ 40 • written 7.7 years ago by
fi1d18
★ 4.2k
8
votes
10
replies
673
views
Inflated GWAS test statistic after merging multiple batches of genotype dataset imputed differently
genotype
imputation
gwas
updated 2 days ago by
LauferVA
3.7k • written 4 days ago by
Amy
▴ 20
799 results • Page
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Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Comment: Deepvariant variant calling by singularity
by
jeffhsu3
• 0
I am having a similar issue was this ever resolved? Getting this error: I0922 22:22:08.794076 140356687390528 make_examples_core.py:257] O…
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