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183 results • Page
2 of 4
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Votes
Replies
2
votes
4
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193
views
Snakemake alignment script
snakemake
alignment
19 hours ago by
Begonia_pavonina
▴ 110
2
votes
13
replies
1.5k
views
RepeatMasker error when trying to generate repeat sequence distribution pie chart (all code and errors provided)
repeatmasker
updated 3 days ago by
Ram
40k • written 6 weeks ago by
epianalysis
• 0
2
votes
5
replies
374
views
ATAC-seq troubleshoot - Just Noise
ATAC-seq
updated 4 days ago by
ATpoint
77k • written 8 days ago by
vk
▴ 40
2
votes
4
replies
3.8k
views
Is there a way to use CNVkit without a reference from normal samples?
sequence
CNVkit
updated 1 day ago by
Matteo Schiavinato
★ 3.6k • written 5.5 years ago by
leandro.bispo.oliveira
• 0
2
votes
4
replies
275
views
Which function is best for pathway analysis?
pathway
2 days ago by
Chris
▴ 200
2
votes
4
replies
269
views
Integrated Seurat object change name of the two conditions
R
integrated
Seurat
4 days ago by
camillab.
▴ 140
2
votes
1
reply
140
views
TPM RNA-seq data for differential expression analysis
RNA-seq
updated 19 hours ago by
Ram
40k • written 23 hours ago by
Catalin-George
• 0
2
votes
0
replies
158
views
Job:
Human Genomics Team Leader
ebi
ensembl
embl
genomics
4 days ago by
Ben_Ensembl
★ 2.3k
2
votes
8
replies
502
views
Read block operation failed with BAM file
bgzip
samtools
bam
pysam
htslib
updated 4 days ago by
John Marshall
3.0k • written 11 days ago by
Alex Reynolds
35k
2
votes
4
replies
304
views
Using STAR aligner to build index of hg38
hg38
STAR
alignment
RNAseq
index
6 days ago by
Grace
• 0
2
votes
4
replies
223
views
Automate the Splitting of a VCF File by Sample (bcftools)
bcftools
vcf
updated 51 minutes ago by
Pierre Lindenbaum
157k • written 1 day ago by
RogueBiochemist
• 0
2
votes
9
replies
472
views
Calculation of TMB on gene level
genomics
updated 4 days ago by
svp
▴ 590 • written 5 days ago by
smrutimayipanda
▴ 20
2
votes
2
replies
254
views
Simulate short-read RNA-seq data from long-read RNA-seq data
polyester
single-cell
simulation
short-read
long-read
3 days ago by
rhonddaskl
• 0
2
votes
6
replies
300
views
obtaining circular RNAs' sequences from circBase
circular-RNA
2 days ago by
aminijavad4
▴ 10
2
votes
5
replies
304
views
How to change from gene_id to GeneID/EntrezID using R
identifiers
Deseq2
GeneID
updated 5 days ago by
Kevin Blighe
86k • written 6 days ago by
Nicolas
• 0
2
votes
1
reply
222
views
News:
Successful NCBI NIAID Codeathon on VCF Files in SARS-CoV-2 Genomics
NCBI
Codeathon
updated 5 days ago by
chrchang523
10k • written 8 days ago by
PeterC_NCBI
▴ 330
2
votes
10
replies
2.8k
views
6 follow
Split plink files by a number of SNPs
GWAS
SNP
plink
updated 1 day ago by
Raygozak
★ 1.4k • written 5.2 years ago by
kakukeshi
▴ 80
2
votes
5
replies
1.8k
views
How to make alpha diversity boxplot?
alpha_diversity
taxa
ASV
box_plot
shannon
updated 5 days ago by
yeah
• 0 • written 10 months ago by
Amr
▴ 140
2
votes
4
replies
187
views
Subclustering of intergated cells from scRNA-seq data
scRNA-seq
Seurat
8 hours ago by
fifty_fifty
▴ 60
2
votes
2
replies
211
views
Get support value of MRCA given a list of tip.label in R newick trees
phylogeny
newick
R
ape
tree
1 day ago by
pl.terzian
• 0
2
votes
5
replies
393
views
6 follow
How to convert data in r?
R
offtopic
updated 19 hours ago by
Ram
40k • written 1 day ago by
star
▴ 350
2
votes
5
replies
276
views
Mapping SNP rsIds/ positions to genes coding for proteins
SNP
rsid
1 day ago by
Harsh
• 0
1
vote
5
replies
275
views
CollectRnaSeqMetrics (Picard) output to convert FeatureCounts into TPM
R
Picard
CollectRnaSeqMetrics
updated 1 day ago by
Ram
40k • written 2 days ago by
camillab.
▴ 140
1
vote
2
replies
279
views
Dotplot error: subscript out of bound
R
scRNA-seq
seurat
6 days ago by
Xuhao
• 0
1
vote
11
replies
789
views
Quantification after transcriptome assembly with Trinity
De-novo-transcriptome-assembly
Trinity
RNA-seq-analysis
updated 5 days ago by
GenoMax
134k • written 4 weeks ago by
langziv
▴ 50
1
vote
2
replies
279
views
Restricting vcf entries based on ID length
bcftools
vcf
vcftools
updated 5 days ago by
chrchang523
10k • written 22 days ago by
ethan.kreuzer
• 0
1
vote
1
reply
994
views
SCENIC-openloom not working
sc-rna
SCENIC
sequencing
updated 5 days ago by
kyriakds
• 0 • written 2.1 years ago by
Lekshmy
▴ 10
1
vote
3
replies
282
views
How to find out what adapters to remove after FastQC of RNAseq data?
Trimmomatic
FastQC
NGS
RNA-seq
5 days ago by
ella
• 0
1
vote
2
replies
156
views
Is it normal if regress out the cell cycle effects but the DEGs are quite the similar (no big changes)
regress_out
Cell_cycle
Seurat
4 days ago by
alwayshope
▴ 30
1
vote
1
reply
234
views
bedGraph for Coverage of Insert from Paired-End Data?
genome
awk
coverage
bed
paired-end
updated 4 days ago by
shelkmike
▴ 980 • written 7 days ago by
vanbelj
▴ 40
1
vote
3
replies
416
views
I have performed Trimmomatic run for adapter removal. QC report shows drop in the reads and presence of overrepresented sequences. Seeking help!
NGS
bioinformatics
illumina
WGS
updated 4 days ago by
swbarnes2
13k • written 5 days ago by
Vijith
▴ 30
1
vote
3
replies
243
views
miRDeep2 - identifying miRNAs from deep sequencing data
microRNA
miRDeep2
updated 4 days ago by
Ram
40k • written 4 days ago by
Hasan
• 0
1
vote
4
replies
259
views
How to sort cd-hit-est cluster file
sort
cd-hit-est
4 days ago by
Mo
▴ 40
1
vote
2
replies
256
views
Understanding TCGA barcodes with dot in the fieldname
tcga
updated 4 days ago by
Zhenyu Zhang
▴ 980 • written 7 days ago by
Tahsin
• 0
1
vote
2
replies
726
views
Job:
Scientist Position, Data Science AI/ML for Biology (JRD DS)
Data-Science
JNJ
Janssen
updated 4 days ago by
Ram
40k • written 14 months ago by
Shicheng Guo
★ 9.3k
1
vote
4
replies
273
views
geom_signif() uses t-test to compare between more than 3 groups... Isn't this wrong?
ggplot2
ANOVA
t-test
4 days ago by
RM123
▴ 10
1
vote
5
replies
285
views
Enrichment of mitochondrial and ribosomal pathways - an artifact?
Pathway
Mitochondria
Ribosome
scRNA-seq
Enrichment
3 days ago by
omer.shomrat
• 0
1
vote
8
replies
331
views
How to plot proportion of cells in each cluster with scanpy?
scRNA-seq
scanpy
single-cell
3 days ago by
bioinfo
▴ 110
1
vote
5
replies
219
views
bcftools error merging two VCFs: REF prefixes differ
bcftools
VCF
3 days ago by
Shane
• 0
1
vote
4
replies
245
views
Running Rockhopper from a command-line
rockhopper
java
3 days ago by
langziv
▴ 50
1
vote
3
replies
610
views
module and trait correlation for WGCNA
treatment
WGCNA
relation
microarray
module-treatment
updated 3 days ago by
andres.firrincieli
3.5k • written 6 months ago by
Shriyansh
• 0
1
vote
2
replies
294
views
OMA in AWS cloud
OMA
AWS
updated 2 days ago by
Adrian Altenhoff
★ 1.1k • written 8 days ago by
Ksel
• 0
1
vote
4
replies
220
views
How to filter vcf file by MAF using bcftools?
bcftools
vcf
updated 2 days ago by
Ram
40k • written 2 days ago by
_quantum_girl_
▴ 10
1
vote
9
replies
383
views
how to identify uniq genes between two gff files.
Genes
Uniq
Annotation
GFF
2 days ago by
nikhil
▴ 20
1
vote
1
reply
157
views
Is it possible to convert 3 prime sequencing read counts into TPMs?
RNAseq
TPM
expression
updated 2 days ago by
Trivas
★ 1.5k • written 2 days ago by
Mat
▴ 60
1
vote
1
reply
161
views
Transcription factor co-localization p-values
p-values
updated 2 days ago by
jared.andrews07
★ 15k • written 2 days ago by
Vishal
▴ 10
1
vote
2
replies
389
views
The bam file used for DiffBind?
DiffBind
ATAC-seq
2 days ago by
Miao Zhang
• 0
1
vote
3
replies
318
views
How to determine the total count for each gene in lymphotype B
scRNAseq
Seurat
1 day ago by
dalibenam64
• 0
1
vote
5
replies
490
views
Number of accessible regions included by DESeq2 to generate the PCA plot with the nf-core ATACseq pipeline ?
nf-core
atac-seq
PCA
DESeq2
updated 1 day ago by
Michael Love
★ 2.5k • written 2 days ago by
Alexandre Eeckhoutte
• 0
1
vote
3
replies
239
views
comparision of umap single cell
single-cell
1 day ago by
synat.keam
▴ 80
183 results • Page
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Recent Votes
Comment: ncbi error report log for validate fastq issue
Comment: ncbi error report log for validate fastq issue
A: Bowtie2 MAPQ difference using local vs end-to-end alignment
C: Bowtie2 MAPQ difference using local vs end-to-end alignment
Answer: TPM RNA-seq data for differential expression analysis
A: Error When Installing Emboss
Answer: Subclustering of intergated cells from scRNA-seq data
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Recent Replies
Comment: Getting the overlap between two GTF files
by
GenoMax
134k
Something in `AGAT` should work: https://agat.readthedocs.io/en/latest/?badge=latest
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Pierre Lindenbaum
157k
> My problem has been with trying to loop the process. ok here is a **nextflow** based solution,(NOT tested) workflow { …
Comment: Assessing Rockhopper's output
by
langziv
▴ 50
I see. I tried using the GUI Rockhopper for RNA-seq analysis of a K. pneumoniae strain, but the program didn't find the strain's assembly…
Answer: Getting the overlap between two GTF files
by
Pierre Lindenbaum
157k
bedtools intersect \ -a <(awk '/^[^#]/ {printf("%s\t%d\t%s\t%s\n",$1,int($4)-1,$5,$0);}' file1.gtf | sort -t $'\t' -k1,1 -k2…
Comment: Assessing Rockhopper's output
by
shelkmike
▴ 980
I used Rockhopper only for bacterial genomes assembled into circular contigs or circular scaffolds.
Comment: Unable to install HorvathMammalMethylChip40manifest packages
by
ATpoint
77k
There is no package of that name in CRAN or Bioconductor. Where do you have it from, so which tutorial you follow?
Comment: Subclustering of intergated cells from scRNA-seq data
by
fifty_fifty
▴ 60
thank you. This is exactly what I was looking for
Answer: Search RCSB with a list of protein names?
by
Mensur Dlakic
★ 25k
This is not the most elegant solution, but it should work with previous suggestions. In this remote directory: http://ftp.wwpdb.org/p…
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Ram
40k
The manual says you can split samples out at once using the `+split` plugin. So, `bcftools +split -Oz -o <PATH> file.vcf.gz` should do the …
Comment: differences between trajectories in conditions with Monocle3 or other tools
by
Amitm
★ 2.2k
Phate (or Monocle) doesn't do UMAP. If you have done UMAP already (using seurat) then you could use the cell barcode to UMAP cluster label …
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I figured out how to make it work. The bams have to be comma separated not just by space: cd "${input_directory_with_associated_bam_fi…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
Hey, this is so close to what I'm looking for - I clarified in my edit. This is a great way to batch download lots of PDBs in RCSB by their…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
This is a really neat package, but I don't think it does exactly what I want, so I've clarified my question. This seems like it grabs a PD…
Comment: How to get the gft file to run velocyto for velocity analysis?
by
Chris
▴ 200
Yes, thank you! I figured it out but I got an error: velocyto run10x -m Hg38_rmsk.gtf WT/outs /cellranger/reference/refdata-cellranger…
Comment: How to get the gft file to run velocyto for velocity analysis?
by
bk11
★ 1.3k
You can get from here- [UCSC][1]. Just click on get output botton. [1]: https://genome.ucsc.edu/cgi-bin/hgTables?hgsid=611454127_NtvlaW…
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