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113,891 results • Page
2 of 2278
Sort: Votes
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Views
Votes
Replies
149
votes
35
replies
61k
views
33 follow
Tutorial:
Tools For Metagenomic Data Analysis
metagenomics
next-gen
updated 9 months ago by
Ram
41k • written 11.0 years ago by
vijay
★ 1.6k
148
votes
70
replies
48k
views
20 follow
Tutorial:
Network plot from expression data in R using igraph
microarray
RNA-Seq
R
network
updated 3 months ago by
Raheleh
▴ 260 • written 6.0 years ago by
Kevin Blighe
86k
146
votes
31
replies
133k
views
26 follow
Useful Bash Commands To Handle Fasta Files
command-line
fasta
bash
updated 4 months ago by
Ram
41k • written 11.8 years ago by
Anima Mundi
★ 2.9k
146
votes
34
replies
53k
views
17 follow
Tutorial:
Working with MAF files (Mutation Annotation Format) from the TCGA (The Cancer Genome Atlas)
music
cancer
tcga
mutation
maf
updated 9 months ago by
Ram
41k • written 10.6 years ago by
Cyriac Kandoth
6.0k
145
votes
56
replies
8.3k
views
17 follow
Forum:
Suggesting The Removal Of Post Closing
biostars
updated 9 months ago by
Ram
41k • written 10.7 years ago by
Istvan Albert
99k
144
votes
62
replies
11k
views
23 follow
Forum:
Is The Biostar Fading? (Updated For 2014)
meta
biostars
updated 9 months ago by
Ram
41k • written 12.7 years ago by
Joachim
★ 2.9k
144
votes
14
replies
21k
views
15 follow
What Are The Classic Papers In Bioinformatics?
literature
papers
history
updated 2.7 years ago by
Jeremy Leipzig
22k • written 13.1 years ago by
Casey Bergman
18k
143
votes
33
replies
29k
views
20 follow
C/C++ Libraries For Bioinformatics?
c
c
updated 4.6 years ago by
chernouhov sergey
▴ 50 • written 13.5 years ago by
User 59
13k
142
votes
13
replies
23k
views
Alignment and mapping
alignment
next-gen
mapping
updated 7.7 years ago by
Benn
8.3k • written 7.7 years ago by
statfa
▴ 740
142
votes
21
replies
29k
views
12 follow
Tutorial:
A few words for R beginners
Swirl
R
updated 19 months ago by
Ram
41k • written 9.0 years ago by
Parham
★ 1.6k
142
votes
14
replies
167k
views
16 follow
What Is The Sequencing 'Depth' ?
next-gen
sequencing
updated 21 days ago by
Ram
41k • written 13.7 years ago by
Pierre Lindenbaum
158k
138
votes
21
replies
70k
views
18 follow
Database Of Tumor Suppressors And/Or Oncogenes
cancer
gene
database
oncogene
tumor
3.2 years ago by
Malachi Griffith
19k
138
votes
42
replies
21k
views
22 follow
Forum:
Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
meta
updated 8 months ago by
LauferVA
3.8k • written 10.5 years ago by
Whetting
★ 1.6k
137
votes
25
replies
81k
views
14 follow
Tutorial:
Piping With Samtools, Bwa And Bedtools
unix
samtools
bwa
bedtools
updated 20 months ago by
Ram
41k • written 11.6 years ago by
Ying W
★ 4.2k
137
votes
33
replies
61k
views
19 follow
VCF files: Change Chromosome Notation
vcf
next-gen
sequence
updated 13 months ago by
Ram
41k • written 9.6 years ago by
Quak
▴ 480
136
votes
26
replies
24k
views
14 follow
Best Way For A Beginner To Get Up To Speed On Unix Quickly?
unix
perl
genomics
updated 9 months ago by
Ram
41k • written 11.9 years ago by
Brett Thomas
▴ 300
136
votes
29
replies
7.2k
views
16 follow
How Do We Discourage Ad-Hoc Bioinformatic Analyses?
software
updated 19 months ago by
Ram
41k • written 12.9 years ago by
Jeremy Leipzig
22k
135
votes
18
replies
165k
views
16 follow
Blast - Formatting Output
blast
format
output
updated 11 months ago by
katieostrouchov
▴ 30 • written 10.0 years ago by
timjoncooper
▴ 320
134
votes
65
replies
5.2k
views
11 follow
Forum:
Bioinformatics vs. the Climate Crisis
RNA-Seq
Climate
updated 5 months ago by
Ram
41k • written 4.0 years ago by
sviatoslav.kendall
▴ 880
134
votes
27
replies
43k
views
14 follow
Why Are There Many Rna-Seq Hits To Intronic Regions?
rna-seq
updated 19 months ago by
Ram
41k • written 11.6 years ago by
lh3
33k
134
votes
22
replies
59k
views
15 follow
How do I explain the difference between edgeR, LIMMA, DESeq etc. to experimental Biologist/non-bioinformatician
gene
LIMMA
edgeR
updated 6 months ago by
Kevin Blighe
86k • written 6.0 years ago by
Mike
★ 1.9k
132
votes
123
replies
34k
views
23 follow
Tutorial:
Survival analysis with gene expression
cox
gene-expression
survival-analysis
geo
updated 8 months ago by
1769mkc
★ 1.1k • written 5.1 years ago by
Kevin Blighe
86k
131
votes
48
replies
15k
views
19 follow
Justifying Learning Linux For Bioinformatics
linux
subjective
updated 12.3 years ago by
Guangchuang Yu
★ 2.6k • written 12.3 years ago by
Eric Normandeau
11k
130
votes
44
replies
31k
views
16 follow
Forum:
Trimming adapter sequences - is it necessary?
NGS
adapter
RNA-Seq
updated 8 months ago by
Ram
41k • written 7.2 years ago by
Lars
★ 1.0k
130
votes
37
replies
13k
views
27 follow
What Are The Obsolete Research Topics In Bioinformatics?
updated 12.2 years ago by
Pals
★ 1.3k • written 12.3 years ago by
Sirus
▴ 820
130
votes
19
replies
178k
views
13 follow
What'S The Difference Between Cds And Orf?
cds
orf
updated 2.6 years ago by
kajumi
• 0 • written 11.5 years ago by
shiy05
▴ 360
129
votes
32
replies
127k
views
29 follow
Is There A Free Alternative To Ingenuity Pathway Analysis?
network
pathway
updated 20 months ago by
Ram
41k • written 12.7 years ago by
Gon
▴ 540
129
votes
38
replies
7.2k
views
21 follow
Forum:
Who are the Greatest Bioinformaticians Of All Time (GBOAT)
bioinformatics
updated 6 months ago by
Ram
41k • written 4.7 years ago by
Istvan Albert
99k
128
votes
24
replies
81k
views
18 follow
Sequence Length Distribution From A Fastq File
sequence
length
fastq
updated 4.6 years ago by
LuckyLuck
• 0 • written 10.5 years ago by
deepthithomaskannan
▴ 370
128
votes
83
replies
12k
views
8 follow
Tool:
Lossless ALAPY Fastq Compressor (now for MacOS X with 10-20% improved speed and compression ratio)
lossless-fastq-compression
fastq-compressor
updated 5 months ago by
Ram
41k • written 6.7 years ago by
Petr Ponomarenko
★ 2.7k
128
votes
19
replies
28k
views
9 follow
Could you explain the difference between STAR, KALLISTO, SALMON etc. to experimental Biologist/non-bioinformatician
RNA-Seq
alignment
next-gen
R
assembly
updated 2.6 years ago by
Ram
41k • written 4.2 years ago by
WUSCHEL
▴ 720
125
votes
46
replies
47k
views
21 follow
Forum:
Visualization of ChIP-seq data using Heatmaps (Updated: 06/10/16)
visualization
ChIP-Seq
homer
ngsplot
deeptools
updated 8 months ago by
Ram
41k • written 7.7 years ago by
Sinji
★ 3.2k
124
votes
29
replies
6.9k
views
18 follow
What License Do You Use When You Release Code And Data?
software
general
subjective
updated 24 days ago by
Ram
41k • written 13.7 years ago by
Simon Cockell
7.4k
124
votes
13
replies
168k
views
13 follow
How Can I Convert Bam To Sam?
bam
next-gen-sequencing
sam
updated 12 weeks ago by
Ram
41k • written 13.4 years ago by
Biomed
4.9k
122
votes
17
replies
73k
views
8 follow
What Are Phased And Unphased Genotypes?
genotyping
genome
updated 20 months ago by
Ram
41k • written 12.6 years ago by
Nick
▴ 370
122
votes
44
replies
10k
views
24 follow
News:
Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta
biostars
updated 5 weeks ago by
Ram
41k • written 11.0 years ago by
Istvan Albert
99k
121
votes
35
replies
41k
views
20 follow
Forum:
Snakemake vs. Nextflow: strengths and weaknesses
snakemake
nextflow
updated 6 months ago by
Ram
41k • written 6.5 years ago by
ropolocan
▴ 810
121
votes
44
replies
20k
views
21 follow
What Is Your Favorite Question To Ask When Interviewing Potential Bioinformaticians?
subjective
updated 9 months ago by
Ram
41k • written 13.5 years ago by
Madelaine Gogol
5.3k
121
votes
56
replies
97k
views
24 follow
How to plot coverage and depth statistics of a bam file
bam
coverage
depth
next-gen-sequencing
updated 8 weeks ago by
William
▴ 20 • written 9.5 years ago by
kay
▴ 340
120
votes
8
replies
79k
views
GRCh37/38(NCBI) vs hg19/hg38(UCSC)
ncbi
ucsc
grch38
hg38
updated 23 months ago by
Ram
41k • written 9.2 years ago by
pwg46
▴ 520
120
votes
33
replies
38k
views
24 follow
Forum:
Where To Look For Quality Bioinformatics Short Courses And Workshops?
Courses
updated 6 months ago by
carlopecoraro2
★ 2.4k • written 10.3 years ago by
Eric Normandeau
11k
119
votes
25
replies
121k
views
12 follow
Where Can I Download Human Reference Genome In Fasta Format? Hgref.Fa File
human
fasta
sequence
bwa
updated 6.8 years ago by
Hajk-Georg Drost
▴ 160 • written 13.4 years ago by
Biomed
4.9k
119
votes
7
replies
113k
views
Bam And Indexed Bam Files
bam
index
11.9 years ago by
Sahel
▴ 260
119
votes
23
replies
30k
views
14 follow
Why are we still using Bam files? And not Cram, HDF5 or improved Bam files?
cram
bam
io
hdf
updated 2.7 years ago by
Ram
41k • written 9.4 years ago by
WilliamS
▴ 320
118
votes
29
replies
32k
views
11 follow
PCA in a RNA seq analysis
RNA-Seq
pca
updated 5.0 years ago by
Giovanni M Dall'Olio
28k • written 6.1 years ago by
Mozart
▴ 330
118
votes
31
replies
191k
views
17 follow
Correct Way To Parse A Fasta File In Python
python
fasta
updated 18 days ago by
Ram
41k • written 13.6 years ago by
Eric Normandeau
11k
118
votes
15
replies
103k
views
15 follow
Economics Of A Career In Bioinformatics
career
updated 12.9 years ago by
Mona Mohamed
• 0 • written 13.1 years ago by
Terence
▴ 210
118
votes
74
replies
44k
views
40 follow
Is there a Lims that doesn't suck?
LIMS
updated 13 months ago by
GenoMax
136k • written 13.0 years ago by
Casbon
★ 3.3k
118
votes
26
replies
34k
views
22 follow
Transcript to gene level count for DEseq(2) use- Salmon/Sailfish/Kallisto etc.
DESeq
Kallisto
RNASeq
Salmon
DESeq2
updated 8 months ago by
e.r.zakiev
▴ 170 • written 8.5 years ago by
mhockin
▴ 610
117
votes
66
replies
44k
views
20 follow
Tutorial:
[Deprecated] Fast download of FASTQ files from the European Nucleotide Archive (ENA)
aspera
fastq-dump
Fastq
ENA
updated 8 months ago by
Ram
41k • written 5.4 years ago by
ATpoint
78k
113,891 results • Page
2 of 2278
Recent Votes
Comment: Problematic fastq files...How can we trust them?
Answer: GO categorization
Comment: Using metagenome assembly and binning to identify and mitigate contamination in
Answer: How to obtain data on the coordinates of the Exon region from UCSC
Answer: How to obtain data on the coordinates of the Exon region from UCSC
Answer: How to obtain data on the coordinates of the Exon region from UCSC
Answer: An incomprehensible error with R package gggenes
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seta
★ 1.8k
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endre.sebestyen
▴ 10
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▴ 560
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Recent Replies
Comment: Yes .. BBMap can do that! - Part III clumpify (mark (and dedupe) duplicates with
by
GenoMax
136k
Yes that should be true. I will let https://www.biostars.org/u/14684/ confirm.
Comment: From TPM to raw counts
by
Gama313
▴ 120
Thanks for the answe and the linkI used bioinfokit tpm formula to calculate tpm from bulk which is the same formula given in your link: A= …
Comment: From TPM to raw counts
by
Gama313
▴ 120
Thanks Brian for the suggestion. However, I did the whole process, from bulk counts generation, to data transformation and scrna deconvolut…
Answer: Are 10x cellranger-arc ATAC bam files deduplicated?
by
swbarnes2
13k
My experience with regular 10x bam files is that duplicates are not removed, but they are flagged as duplicates. So take a look at the …
Comment: Can I use TCGA-LUAD RNAseq count that had already normalized by RSEM in Limma-vo
by
fluke
• 0
Thanks a lot for your answer, I’m confused between RSEM expected count and RSEM normalized count. I’m working on this dataset from UCSC ht…
Answer: From TPM to raw counts
by
Istvan Albert
99k
In principle, the TPM formula can be reverted, see the timeless post * [What the FPKM? A review of RNA-Seq expression units][1] In p…
Comment: Low coverage whole genome sequencing reveal excess heterozygosity for multiple S
by
beausoleilmo
▴ 560
You're right, I wasn't explaining the problem clearly. Thanks for the directions! - The depth; coverage ~3.00X ± 2.50 SD - The sequen…
Comment: Using metagenome assembly and binning to identify and mitigate contamination in
by
Mensur Dlakic
★ 25k
All good points, especially about multiple copies of single-copy genes. I am doing error-correction in my assemblies, but was making an edu…
Comment: Can I use TCGA-LUAD RNAseq count that had already normalized by RSEM in Limma-vo
by
CTLong
▴ 20
Yes, normalized RSEM counts from TCGA can be used as input for Limma Voom. Please check this post https://support.bioconductor.org/p/63981/…
Comment: How to obtain data on the coordinates of the Exon region from UCSC
by
ayasu
• 0
Sorry for the delay in expressing my thanks. I found the advice to look at the information from MySQL very useful. I will also refer to t…
Comment: From TPM to raw counts
by
Brian Bushnell
19k
I'm posting this as a comment instead of an answer specifically because it's just what I would do and I don't know if it's the best approac…
Comment: Using metagenome assembly and binning to identify and mitigate contamination in
by
Brian Bushnell
19k
In most cases error-correction should take care of error-spawned fake minor alleles, though... > If you want to convince yourself of this,…
Answer: Using metagenome assembly and binning to identify and mitigate contamination in
by
Mensur Dlakic
★ 25k
It is a valid question, and I particularly like when posters err on the side of providing more than less detail. Metagenomic binning can be…
Answer: Low coverage whole genome sequencing reveal excess heterozygosity for multiple S
by
Brian Bushnell
19k
I don't understand your plot. Perhaps a legend would help? I also don't know what you mean by "genotype frequency"; is that the ratio of …
Comment: Does GNOMAD use all LOFTEE LoF filters?
by
AMARU
• 0
Can you post the commands you used? I am having some issues running that plugin on vep v110. It appears in fields but it doesn't annotat…
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