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799 results • Page
2 of 16
Sort: Rank
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0
votes
1
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177
views
Esearch, Epost, and Efetch for Large Datasets in Biopython
eutils
biopython
entrez
updated 2 days ago by
Ram
40k • written 2 days ago by
Salem
• 0
1
vote
2
replies
224
views
GSEA Result Visualization in R
r
enrichplot
gsea
visualization
enrichResult
2 days ago by
Cortney
• 0
1
vote
2
replies
244
views
How to separate proteins on the same chain?
pdb
protein
updated 2 days ago by
Ram
40k • written 3 days ago by
Zayyan
▴ 10
0
votes
2
replies
213
views
how to evaluate SNPs that are regulating same gene expression across multiple tissues
snp
GTEx
updated 3 days ago by
LChart
3.3k • written 3 days ago by
rheab1230
▴ 140
3
votes
10
replies
434
views
STAR index not working
STAR
RNA-Seq
1 day ago by
camillab.
▴ 130
1
vote
2
replies
358
views
How to display all top markers in the heatmap by DoHeatmap (Seurat) when there are duplicates of top marker genes for several cell clusters
DoHeatmap
Seurat
2 days ago by
alwayshope
▴ 30
2
votes
3
replies
258
views
Can I infer the fraction of replicating cells from bulk RNA-seq data?
replication
apoptosis
updated 3 days ago by
Ram
40k • written 3 days ago by
txema.heredia
▴ 80
0
votes
1
reply
149
views
Prank checkpoint and restore?
prank
alignment
updated 3 days ago by
Ram
40k • written 3 days ago by
Pit
• 0
0
votes
0
replies
547
views
Job:
Research Scientist 5 – Bioinformatics and Computer Biology, Center for Dementia Research (CDR), Nathan Kline Institute for Psychiatric Research
Neuroscience
Biostatistics
updated 3 days ago by
Ram
40k • written 3 days ago by
cdrinfo
• 0
0
votes
1
reply
167
views
gffread outputs empty gtf file
gff
gffread
gtf
updated 3 days ago by
inedraylig
▴ 20 • written 3 days ago by
sumitra.20
• 0
0
votes
13
replies
468
views
STAR Intron Motif Script Gives Segmentation fault Error
STAR
Linux
2 hours ago by
Y
• 0
5
votes
7
replies
436
views
Elusive syntax error in Snakefile
snakemake
updated 3 days ago by
Ram
40k • written 4 days ago by
Giuseppe Giovanni
▴ 30
1
vote
3
replies
216
views
Sub-sampleing bam files based on sequencing_summary.txt (guppy output)
samtools
guppy
updated 3 days ago by
GenoMax
134k • written 3 days ago by
anika.john
• 0
8
votes
10
replies
673
views
Inflated GWAS test statistic after merging multiple batches of genotype dataset imputed differently
genotype
imputation
gwas
updated 2 days ago by
LauferVA
3.7k • written 4 days ago by
Amy
▴ 20
2
votes
13
replies
579
views
Dealing with transcriptome sequences that are smaller than their respective genes
blastn
Trinity
Transcriptome
RNA-seq-analysis
De-novo-transcriptome-assembly
1 day ago by
langziv
▴ 50
2
votes
2
replies
188
views
Kraken2 recovering only classified reads
Kraken2
taxonomy
classification
3 days ago by
SushiRoll
▴ 110
0
votes
0
replies
104
views
Any way to predict hormone biosynthesis pathways in understudied organisms?
genomics
orthologs
biosynthesis-pathway
updated 3 days ago by
Ram
40k • written 3 days ago by
Mark
• 0
2
votes
4
replies
288
views
Refseq annotation | Populus trichocarpa
R
RNA-seq
plants
Refseq
updated 3 days ago by
Ram
40k • written 3 days ago by
P
• 0
1
vote
1
reply
177
views
Read Counts from BAM file
bam
samtools
qualimap
readcount
updated 3 days ago by
ATpoint
76k • written 3 days ago by
Smriti
• 0
10
votes
8
replies
459
views
Adding a control sample to bulk RNA-seq
RNA-seq
1 day ago by
Chris
▴ 180
19
votes
14
replies
793
views
Forum:
What is the amount of sequencing data produced annually?
data
research
sequencing
updated 2 days ago by
Jeremy Leipzig
21k • written 3 days ago by
vincenthus
▴ 70
0
votes
2
replies
217
views
Is it okay to just average the log2FC values across different cell types in pseudobulk scRNA-seq data?
scrna-seq
rna-seq
log2fc
3 days ago by
JLee
• 0
1
vote
3
replies
301
views
VDJ reconstitution from 3' 10x genomics platforms
VDJ
updated 3 days ago by
Ram
40k • written 4 days ago by
gal.dadi
• 0
0
votes
1
reply
168
views
Identify parent of each read in a GAF
gaf
vgteam
vg
updated 3 days ago by
Jordan M Eizenga
▴ 360 • written 4 days ago by
cfourps
▴ 10
0
votes
3
replies
225
views
Errors while trying to run Scenic
Scenic
updated 3 days ago by
GenoMax
134k • written 4 days ago by
Stavroula
• 0
0
votes
3
replies
241
views
How to find target genes From RNA seq data?
Cotton
DGE
RNA-seq
updated 4 days ago by
Ram
40k • written 4 days ago by
Fizzah
▴ 30
1
vote
2
replies
204
views
STAR error EXITING because of FATAL ERROR: failed reading from temporary file
STAR
RNA-seq
updated 4 days ago by
GenoMax
134k • written 4 days ago by
erik.burchard
▴ 20
2
votes
3
replies
242
views
Is there an easy to modify the subplot titles in DimPlot (Seurat)?
scRNA-seq
patchwork
DimPlot
Seurat
updated 3 days ago by
Nicolas Rosewick
10k • written 4 days ago by
alwayshope
▴ 30
0
votes
0
replies
120
views
News:
Course: Data Manipulation and Visualisation with Python
Python
Data-Visualization
Data-Manipulation
updated 4 days ago by
Ram
40k • written 4 days ago by
carlopecoraro2
★ 2.3k
0
votes
1
reply
229
views
VCF QUAL field for multiple samples
variants
VCF
updated 4 days ago by
DBScan
▴ 180 • written 12 days ago by
Ron
• 0
0
votes
3
replies
479
views
CNV calling
CNV
NGS
Variant-Calling
updated 3 days ago by
Zhenyu Zhang
▴ 980 • written 8 months ago by
adarsh_pp
▴ 30
2
votes
1
reply
209
views
bedGraphToBigWig: Missing Genome Coordinates
bamtobed
bed
4 days ago by
vanbelj
▴ 40
0
votes
0
replies
130
views
News:
Online Course - A Practical Introduction to NGS Data Analysis - October 23-25, 2023
DNA-seq
RNA-seq
Workshop
updated 4 days ago by
Ram
40k • written 4 days ago by
David Langenberger
10k
0
votes
0
replies
121
views
maftools - median survival time doesn't match plot
R
survival
maftools
K-M
4 days ago by
BioGuy
• 0
6
votes
8
replies
451
views
Find potential important genes from bulk-RNA seq experiment
RNA-seq
18 hours ago by
Chris
▴ 180
0
votes
1
reply
152
views
vg rna pantranscriptome
rna
vg
updated 4 days ago by
Jordan M Eizenga
▴ 360 • written 4 days ago by
z
• 0
0
votes
0
replies
112
views
Genetic distance in cM from VCF of non-reference species to run Beagle
plink
beagle
vcf
updated 4 days ago by
Ram
40k • written 4 days ago by
AndrMod
• 0
1
vote
4
replies
1.2k
views
ConsensusClusterPlus: How to extract most contributing features for each cluster
R
consensusclusterplus
updated 4 days ago by
LChart
3.3k • written 2.6 years ago by
komal.rathi
★ 4.1k
1
vote
4
replies
266
views
rRNA filtering from human RNA-seq data
rRNA
RNA-seq
4 days ago by
deniz
▴ 10
0
votes
5
replies
321
views
manhattan plot with vcf information
R
manhattan
vcf
updated 2 days ago by
dthorbur
▴ 550 • written 4 days ago by
sooni
▴ 10
6
votes
1
reply
142
views
How to retrieve sample informations from given ID from Sequence Read Archives?
eutils
ncbi
SRA
updated 4 days ago by
Ram
40k • written 4 days ago by
DareDevil
★ 3.3k
2
votes
6
replies
370
views
Read block operation failed with BAM file
bgzip
samtools
bam
pysam
htslib
4 days ago by
Alex Reynolds
35k
0
votes
0
replies
421
views
after gatk VariantAnnotator -V *_com_norm.vcf -A AlleleFraction -O *_norm_AB.vcf There "nan,nan" or "nan" in my vcf file
gatk
AlleleFraction
VariantAnnotator
updated 4 days ago by
Ram
40k • written 4 days ago by
zhuo
• 0
2
votes
0
replies
132
views
the dispersion estimation of edgeR and DESeq2
estimation
dipersion
edgeR
DESeq2
4 days ago by
tommy
▴ 30
6
votes
3
replies
282
views
Create 10,000bp windows for a SNP file and assign each SNP to its respective window
genome
snp
vcf
updated 4 days ago by
Alex Reynolds
35k • written 4 days ago by
nitinra
▴ 50
1
vote
3
replies
255
views
Fastqc loop
fastQC
r
RNA-seq
4 days ago by
camillab.
▴ 130
0
votes
2
replies
552
views
News:
The Practice of RADseq: Population Genomics Analysis with Stacks (RADS02) Early bird deadline approaching - 20th September
population-genomics
radseq
stacks
updated 4 days ago by
Dave Carlson
★ 1.4k • written 4 days ago by
oliverhooker
▴ 110
2
votes
3
replies
268
views
Genewise output help
genewise
updated 4 days ago by
Jean-Karim Heriche
26k • written 4 days ago by
lorenzoedg
• 0
0
votes
1
reply
189
views
Add samples IDs to Seurat object when integrating different samples to do differential expression analysis
scRNA-seq
Seurat
updated 4 days ago by
Ram
40k • written 5 days ago by
Sara
▴ 230
2
votes
1
reply
162
views
Best Tools and Practices for CNV Variant Calling in WES Data
CNV
WES
updated 5 days ago by
Pierre Lindenbaum
157k • written 5 days ago by
Nadav
▴ 10
799 results • Page
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
Comment: Error in bowtie2
Recommended Tools For Alternative Splicing Detection From Rna-Seq Data
Comment: Using STAR aligner to build index of hg38
Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Comment: Deepvariant variant calling by singularity
by
jeffhsu3
• 0
I am having a similar issue was this ever resolved? Getting this error: I0922 22:22:08.794076 140356687390528 make_examples_core.py:257] O…
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