updated just now by
Kevin Blighe
87k
•
written 1 day ago by
qudrat.nii
▴
10
0
votes
3
replies
186
views
0
votes
4
replies
2.6k
views
updated just now by
evawillms183
•
0
•
written 8.3 years ago by
andrepleao
•
0
0
votes
0
replies
30
views
1
vote
3
replies
180
views
updated 2 days ago by
Mensur Dlakic
★
27k
•
written 2 days ago by
emmanouil.a
▴
120
0
votes
1
reply
75
views
updated 3 hours ago by
Pierre Lindenbaum
161k
•
written 6 hours ago by
DKA
▴
40
0
votes
2
replies
111
views
updated 4 hours ago by
Mensur Dlakic
★
27k
•
written 7 hours ago by
biochugs
•
0
795
votes
167
replies
141k
views
109 follow
updated 3 months ago by
Biostar
2.7k
•
written 7.4 years ago by
Istvan Albert
100k
3
votes
6
replies
294
views
0
votes
6
replies
605
views
1
vote
10
replies
826
views
6 hours ago by
DKA
▴
40
0
votes
0
replies
49
views
8 hours ago by
hemantcnaik
•
0
0
votes
2
replies
735
views
0
votes
3
replies
147
views
updated 10 hours ago by
GenoMax
141k
•
written 11 hours ago by
Bertalan_Takacs
▴
90
0
votes
0
replies
67
views
updated 7 hours ago by
Ram
43k
•
written 11 hours ago by
glaciya2018
•
0
0
votes
0
replies
54
views
11 hours ago by
David Langenberger
11k
0
votes
0
replies
97
views
0
votes
14
replies
3.4k
views
6 follow
updated 13 hours ago by
drstalinantony28
•
0
•
written 2.2 years ago by
ymj
▴
10
0
votes
1
reply
94
views
updated 10 hours ago by
Sofia
•
0
•
written 14 hours ago by
mawigoj318
•
0
0
votes
0
replies
76
views
16 hours ago by
salias
•
0
4
votes
5
replies
264
views
0
votes
2
replies
140
views
0
votes
0
replies
69
views
updated 7 hours ago by
Ram
43k
•
written 17 hours ago by
newuser2024
•
0
0
votes
0
replies
59
views
0
votes
1
reply
106
views
updated 7 hours ago by
Ram
43k
•
written 18 hours ago by
ahmad.sajad4541
•
0
0
votes
0
replies
64
views
updated 6 hours ago by
Ram
43k
•
written 19 hours ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
60
views
0
votes
2
replies
170
views
2
votes
2
replies
150
views
updated 21 hours ago by
analyst
▴
30
•
written 1 day ago by
lorena9132
•
0
0
votes
0
replies
74
views
7
votes
2
replies
220
views
updated 1 day ago by
geneontologyhelp
▴
390
•
written 2 days ago by
catherine.teyssier
•
0
0
votes
1
reply
163
views
updated 1 day ago by
Istvan Albert
100k
•
written 2 days ago by
Abeer
•
0
0
votes
4
replies
243
views
0
votes
1
reply
112
views
updated 22 hours ago by
Mensur Dlakic
★
27k
•
written 1 day ago by
benguyarenbeyaz98
•
0
0
votes
2
replies
194
views
updated 6 hours ago by
Ram
43k
•
written 1 day ago by
v.berriosfarias
▴
140
2
votes
1
reply
170
views
1
vote
1
reply
140
views
updated 1 day ago by
Pierre Lindenbaum
161k
•
written 1 day ago by
Biostar
2.7k
1
vote
1
reply
117
views
updated 1 day ago by
GenoMax
141k
•
written 1 day ago by
heelpPlease
•
0
2
votes
9
replies
786
views
0
votes
10
replies
351
views
0
votes
1
reply
126
views
updated 1 day ago by
bk11
★
2.3k
•
written 1 day ago by
alphaflylizard
•
0
3
votes
3
replies
186
views
0
votes
3
replies
204
views
updated 1 day ago by
Istvan Albert
100k
•
written 1 day ago by
Dylan
•
0
1
vote
4
replies
210
views
4
votes
11
replies
466
views
3
votes
13
replies
622
views
0
votes
0
replies
82
views
1 day ago by
ohtang7
▴
40
3
votes
0
replies
101
views
updated 1 day ago by
Ram
43k
•
written 1 day ago by
carlopecoraro2
★
2.5k
2
votes
2
replies
400
views
updated 1 day ago by
Pierre Lindenbaum
161k
•
written 8 months ago by
AshleeThomson
▴
80
0
votes
1
reply
136
views
8
votes
5
replies
4.9k
views
Recent Votes
Recent Locations •
All
China, 2 minutes ago
United States, 5 minutes ago
South Korea, 11 minutes ago
USA, 11 minutes ago
United States, 14 minutes ago
United States, 14 minutes ago
Singapore, 15 minutes ago
Recent Awards •
All
Popular Question to
Simone
▴
10
Popular Question to
carlopecoraro2
★
2.5k
Popular Question to
daffodil
▴
10
Popular Question to
Russ
▴
500
Popular Question to
camillab.
▴
160
Popular Question to
pramach1
▴
40
Popular Question to
Adyasha
•
0
Recent Replies
Comment: clustalo - align milions of short seq, memory issue
by
emmanouil.a
▴
120
Hi GenoMax and Mensur Dlakic, thank you for your feedback. I'm changing approach in the way to work with a smaller number of seq.
Comment: Annovar using R package
by
Pierre Lindenbaum
161k
duplicate of your previous question: https://www.biostars.org/p/9592646/
why using R when there are shell-based tools like VEP ? slivar , …
Comment: Trouble with Pilon installation
by
Lissa Cruz Saavedra
•
0
Hi
I am trying to install the software but I didn't find the way to clone from github. Could you help me with the pipeline of instruction…
Comment: Adding CB tag to bam file
by
swbarnes2
14k
I think both these programs are just adding the same tag to every line. I don't think either cares whether it's called RG or CB
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Even if this was real data, the tiny numbers for the PC1 and PC2 indicate that the differences between the samples are all absolutely tiny.…
Comment: Creating Synthetic Sequences for a ML Model
by
biochugs
•
0
Thanks for the input. I'll continue to think of more ideas that may be achievable.
Comment: PCA plot (Suggestions Needed)
by
Jeremy
▴
890
You could also try kernel PCA, which is a non-linear form of PCA, although this will be more time-consuming.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
DKA
▴
40
I have unstranded RNA-seq data. Please share the tool with me, if you do not mind.
Comment: PCA plot (Suggestions Needed)
by
Mensur Dlakic
★
27k
Everything that @f1f58aed said, plus that there may be no data manipulation that will give you the clusters you expected. Sometimes there a…
Answer: Creating Synthetic Sequences for a ML Model
by
Mensur Dlakic
★
27k
It is a good idea, though I don't see how it would work in practice.
I think you will encounter at least one additional problem on top of …
Answer: PCA plot (Suggestions Needed)
by
Jeremy
▴
890
Right now, the WT samples are clustering together, but you have one mutant sample off by itself, which could be an outlier. How did you sc…
Comment: Adding CB tag to bam file
by
GenoMax
141k
@yokofakun had written this: https://www.biostars.org/p/336965/
https://github.com/ruqianl/appendCB may also be useful.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
GenoMax
141k
Please post information about your tool as a `tools` category post. Don't wait/ask other users to ask you.
Comment: Adding CB tag to bam file
by
Maria
•
0
Sorry for the delay in my answer, I got sidetracked.
I am using
```samtools addreplacerg -r "@RG\tID:$cell" -o $cell.bam -h $LINE```
bu…
Answer: Troubleshooting RNA-seq data with DNA contamination
by
haibol2017
▴
10
We just developed a R package to tackle this problem.
Do you have stranded or unstranded RNA-seq data contaminated with gDNA?
If you wou…
Traffic: 2026 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6