Question: How to calculate percentage identity/error rate from local alignment score?
0
gravatar for crivenster
5.4 years ago by
crivenster50
India
crivenster50 wrote:

Hi,

I have a local alignment on similar sequences using Smith-Waterman algorithm. I have alignment scores generated,how to calculate the percentage identity between the sequences? can it be calculated from the alignment score ? 

Thanks.

ADD COMMENTlink written 5.4 years ago by crivenster50

Unfortunately, it is not possible to precisely compute the identity merely from a score.

ADD REPLYlink written 5.4 years ago by lh331k

What software have you used to calculate the alignment?

ADD REPLYlink written 5.4 years ago by mikhail.shugay3.3k

I have written a small piece of code, implemented Smith-Waterman algorithm in java as i had some issues with Biojava package. But then i wanted to also find the percentage identity between the sequences. The online searches dint give a clear or a definite picture on calculating the percentage identity between two nucleotide sequences.

ADD REPLYlink written 5.4 years ago by crivenster50
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