assigning function to putative/hypothetical proteins

Question

[robjohn7000]

Hi All,

What is the best and current bioinformatics approach to assign functions to putative/hypothetical proteins?

Thanks

Answer 1

[Prakki Rama]

1. You can always choose BLAST to assign function to your unknown sequence. (also can try programs reciprocal blast for better conviction, inparanoid, RSD if query is proteins)
2. Sometimes, based on domains also, the functions can be assigned. (can try interproscan)

A tool called BLAST2GO has some easy to use features.

[Asaf]

+1 for interproscan

[robjohn7000]

Many thanks for your suggestions Prakki