Question: How to make this kind of 3-D plotting for cancer subclones ? (picture attached)
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gravatar for newDNASeqer
5.1 years ago by
newDNASeqer630
United States
newDNASeqer630 wrote:

I am interested in making 3-D plotting like this below picture for illustrating the subclones/clustered mutations of cancer cells. I have exome-seq data and have calculated the VAFs (Variant Allele Frequency), but I have no idea how to do the plotting. Does anyone know what software of package can do this kind of 3-D plotting?

 

3-D plotting

cancer subclone heterogeneity • 2.7k views
ADD COMMENTlink modified 2.6 years ago by Biostar ♦♦ 20 • written 5.1 years ago by newDNASeqer630
3

I question the value of 3D representation in this figure (and more generally). I think you could generate an equally-informative visualization using e.g. R/ggplot2 + facets.

ADD REPLYlink written 5.1 years ago by Neilfws48k

Link to paper: http://www.nature.com/ng/journal/v46/n3/full/ng.2891.html

ADD REPLYlink modified 5.1 years ago • written 5.1 years ago by Christian2.8k
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gravatar for Noushin N
5.1 years ago by
Noushin N550
Baltimore, MD
Noushin N550 wrote:

In the manuscript, they mention generating this setup of plots in adobe Illustrator, from 2D flat plots generated in R. I know this doesn't really point you any off-the-shelf solution :(

"Variant frequencies extracted from the AmpliSeq validation data were plot-
ted as bar charts for all mutations with a minimum coverage of 100× across all sequenced regions of a tumor using the R software package. These charts were converted into three-dimensional bar charts using Adobe Illustrator."

ADD COMMENTlink written 5.1 years ago by Noushin N550

thanks Noushin, but that last sentence is too terse for me to repeat how they did it :(

ADD REPLYlink written 5.1 years ago by newDNASeqer630

I don't know if it will help you make that nice looking figure, but one other possibility is making a 3D bar plot in your software of choice, then adjusting the distance between bins, and customizing color bars, play with bar depth, etc.

ADD REPLYlink written 5.1 years ago by Noushin N550
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gravatar for Chris Miller
5.1 years ago by
Chris Miller20k
Washington University in St. Louis, MO
Chris Miller20k wrote:

I'll put in a plug for our sciClone package, which produces some nice plots, though nothing exactly like you're showing here. https://github.com/genome/sciclone

ADD COMMENTlink written 5.1 years ago by Chris Miller20k

@Chris Miller, thanks for your reply. 

Yes, I've tried to use your sciClone software, and below is what I got from my preliminary attempts using two of my samples. As you see, the clusters are too close, I was wondering if there's any way to change the scale of the X-axis so that I can make the clusters farther apart ? 

Another question with sciClone is: if I have 10 samples (Exome-Seq), how do I feed them into sciClone to analyze how many subclones exist in the samples? - Thanks again

 

 

ADD REPLYlink modified 5.1 years ago • written 5.1 years ago by newDNASeqer630
1

- Are your VAFs scaled between 0 and 100 (percentage) and not between 0 and 1 (fraction)?

- If your data really does look like this, you could modify with the sc.plot2d function in the R code to tweak the xlim and ylim.

ADD REPLYlink modified 5.1 years ago • written 5.1 years ago by Chris Miller20k

Thanks Chris for the reply.

I had fractional VAFs (between 0 and 1) in my files for the sciClone plotting posted above this, however, after I converted them to percentage, I think I got better looking plots. I have uploaded two captured plots for you to see, however, I still need your help with interpreting the plotting.

1. For the clustering plot that shows 3 clusters, I only included two samples, but I have more than 10 samples, how do I tell the sub clonality in all my samples?

2. The tumor coverage plot shows dots clustered below 20, does this mean my sequencing coverage is only about 20X? is this considered low for sub clone analysis in cancer samples? 

please note that I did not do copy number variation analysis, or should I include it with SNPs for sciClone?

Thanks a lot!

ADD REPLYlink modified 5.1 years ago • written 5.1 years ago by newDNASeqer630

1) That's not your sequencing coverage, that's the VAF. Seeing the highest clone at sub-20% suggests that your tumor purity is very low. 

2) Those outlier points (and corresponding strange clusters) would almost certainly be cleaned up by adding copy-number information. You can derive it from exome data using VarScan, cn.mops, or other packages.

ADD REPLYlink written 5.1 years ago by Chris Miller20k
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gravatar for Alex Reynolds
5.1 years ago by
Alex Reynolds28k
Seattle, WA USA
Alex Reynolds28k wrote:

There isn't an isometric projection plotting function in R that I can find. My guess is that you'd have to make it manually, as the authors did.

I'd instead try going with a 2D heatmap (or levelplot, geom_tile, volcano plot, etc.) to show this kind of data. Use multiple colors to categorize data (e.g. blue, yellow, red, etc.) and apply an intensity or saturation adjustment from 0 (no saturation) to 1 (full saturation) for the normalized variant frequency signal. Read `?hsv` in `R` or search on HSV color space in a search engine for more info. 

A heatmap of normalized data would readily communicate relative differences in signal, whereas with the authors' figure, the reader is guess-timating differences based on eyeballing relative plot heights. Further, it isn't immediately clear what differentiates column values; there are no tick marks and no labels along the x-axis, so any trends along that axis are also guess-timated. These choices cause communication problems that make this a less than successful design, IMO.

ADD COMMENTlink written 5.1 years ago by Alex Reynolds28k
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