miRNA Degradome Sequencing Analysis Flow

Question

[biolab]

Dear all,

Could anyone provide me some suggestions on analysis of degradome sequencing data? I would like to know the recent progress and analysis tools in this field. To me knowledge, M Axtell lab developed Cleaveland, which is popular in plant miRNA analysis (Curr Biol 2008, Bioinformatics 2008). B Meyers and P Green labs also performed degradome analysis (Nat Biotech 2008). These studies were quite early, what's recent progress, especially in animals? What bioinformartic tools are most popular now? I believe any of your advices would be much helpful. THANKS!

Answer 1

[Chirag Nepal]

I think there is one paper in NAR, which is co-authored by John Mattick's lab, where they used degradome-seq for mirna target analysis on mouse. I am not aware of new papers.