How does homozygous allele sequences appear in Sequencing data?

Question

[crivenster]

Hi,

I am new to sequencing and mapping reads in NGS data. I am aware of the definition for Homozygous alleles,but how does they appear in sequence. How do they appear in Read data.

Thank you.

Answer 1

[Devon Ryan]

Generally most of the bases covering a position will be the same (whether they mostly match or mismatch the reference depends on whether you're looking at a homozygous WT or homozygous variant position).