Annovar: filtering of deleterious mutations based on human healthy tissue, in place of Human SNPdb

Question

[Sudheer Gupta]

Hi friends,

I am filtering deleterious mutations from VCF file with the ANNOVAR tool, which removes all general variations which are found in SNPdb. My problem is that I have sequencing data (VCF file) both from cancer tissue and healthy tissue. Now I have to filter those variations from cancer tissue which are found in healthy tissue. How can I use these VCF files in ANNOVAR to get patient specific mutations.

Thanks

Sudheer