I installed EMBOSS 18.104.22.168 and primer3 2.3.6 from source on a computer running Kubuntu 14.04, and set the environment variables for eprimer3 like so:
$ export EMBOSS_PRIMER3_CORE=/opt/primer3-2.3.6/src/primer3_core $ export PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/opt/primer3-2.3.6/src/primer3_config
Then I put a DNA sequence in the file
in.pr3 ran this command:
$ eprimer3 -outfile=out.pr3 -sequence=in.pr3
However, this was the output:
Pick PCR primers and hybridization oligos Error: Missing SEQUENCE tag
I cannot for the life of me figure out what the sequence tag is or how I can set it. All of the solutions on Google have failed to resolve the issue. I am led to believe that the problem has to do with incompatible versions, but someone here allegedly got this working.