Question: analysis amplicon miseq
0
gravatar for chenming_sunshine
4.4 years ago by
China
chenming_sunshine0 wrote:

Hi all,I sequenced dozens of genes in tumor-normal samples using truseq custom amplicon kit on Miseq( whit average depth ~1000x). But if I process the data using the somatic variant caller which used in MiSeq's Amplicon Workflow,the results seem to be too rough,and I have no idea to find out the possible germline or somatic mutations.

Anyone has any idea about the pipeline of analysis? And what is the general standard for germline/somatic mutation.

Thanks.

ADD COMMENTlink modified 12 months ago by Biostar ♦♦ 20 • written 4.4 years ago by chenming_sunshine0
0
gravatar for chenbingjie1992
20 months ago by
chenbingjie19920 wrote:

Hi, i meet the same problem as yours. Have you find the analysis pipeline?

ADD COMMENTlink written 20 months ago by chenbingjie19920
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