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9.8 years ago
Hi all,
I sequenced dozens of genes in tumor-normal samples using truseq custom amplicon kit on Miseq (with average depth ~1000x). But if I process the data using the somatic variant caller which used in MiSeq's Amplicon Workflow,the results seem to be too rough,and I have no idea to find out the possible germline or somatic mutations.
Anyone has any idea about the pipeline of analysis? And what is the general standard for germline/somatic mutation.
Thanks.
Hi, i meet the same problem as yours. Have you find the analysis pipeline?