Question: Visualize regulatory network using cytoscape
0
gravatar for kim
4.3 years ago by
kim60
United States
kim60 wrote:

I got some hub genes based on PPI network and then I did promoter analysis based on transcription factor binding sites. Let's see I have a list of 50 hub genes and 200 transcription factors. I am gonna visualize this regulatory network using Cytoscape. However, I don't have any idea including 1) how to make input file for network importing on Cytoscape, and 2) how to construct this network on Cytoscape. What am I supposed to do?

ADD COMMENTlink modified 4.3 years ago by Neilfws48k • written 4.3 years ago by kim60
2

I'd start by reading some Cytoscape documentation. Surely they have information about how to create input files. Sometimes, when people have "no idea", the answer is that you just have to sit down and work through some basics. 

ADD REPLYlink written 4.3 years ago by Neilfws48k
0
gravatar for lkmklsmn
4.3 years ago by
lkmklsmn820
United States
lkmklsmn820 wrote:

Not sure I understand your question completely but I generally "make" the network before loading it into cytoscape. In order to do so I create a table containing a minimum of two columns where column 1 represents node a and column 2 represent node B. This table will encode the network structure. Each row will therefore mark an edge/interaction.

Column1    Column2  

GeneA       GeneB  

GeneA       GeneC  

Would represent the following network  

GeneB---GeneA---GeneC  

 

You can add additional columns with more information about the interaction e.g. correlation coefficient but this is the most simple way. In cytoscape you can import these networks as csv or tab-delimited tables. Once in cytoscape you will have many options to edit this network, e.g. colors...

 

ADD COMMENTlink written 4.3 years ago by lkmklsmn820
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