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13.2 years ago
Travis
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2.8k
Is anyone aware of software that will generate simulated colorspace reads?
wgsim is supposed to do it but the functionality does not seem to work - I an only get standard fastq data as output.
Thanks in advance.
It doesn't work. Whilst it has the option to output colorspace data (-c), the output is still fastq.
Here is a method from Seqanswers (from Nils Homer) that converts FASTQ to .csfasta and .qual: http://seqanswers.com/forums/showthread.php?t=2360
Shouldn't a .csfasta file contain numbers instead of letters? Nils' script only separates the sequence and quality information and converts the quality scores so far as I can see.
Do you enable the -c flag to tell the tool to generate reads in color space?