Entering edit mode
8.9 years ago
gayachit ▴ 200
I am currently working on an assembled eukaryotic genome. The file size is 2.5 GB. I have read that people use genscan for de-novo gene prediction. Currently I am unable to figure out how as genscan does not work well with multifasta and large files. I need to produce a .gff file. I used a perl script that was available i.e
run_genscan_gff.pl. But that script also encounters the same problem of large file. How does everyone else do this prediction using Genscan? Any help would be useful right now.
Did you download the local version of Genscan from here and ran on your data?