I am trying to do the example in this link:
the lambda example, I use this command:
wgs-8.2alpha/Linux-amd64/bin/PBcR -length 500 -partitions 200 -l lambdaIll -s pacbio.spec -fastq pacbio.filtered_subreads.fastq genomeSize=50000 illumina.frg
but It gives me this error;
unning with 40.01948X (for genome size 50000) of lambdaIll sequences (2000974 bp).
Correcting with 50X sequences (2500000 bp).
AMOS or PBDAGCON is required to call consensus and neither was available. Please add either SMRTportal or AMOS to your path and try again
I already have pbdagcon in my path.
Thanks in advance
Update : after installing AMOS It worked