What the index means in illumina RNA-seq data file?
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6.9 years ago
M K ▴ 540

What the index means in illumina RNA-seq data file? Also is it called barcode? and what is the befit of it.

Please I need details about it.

RNA-Seq sequencing next-gen • 5.3k views
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Please stop wasting people's time by asking several trivial questions in a row. You need to make an effort yourself. Keep reading the definitions and materials that are easily available on the web. If you don't understand it, wait an hour and read it again.

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Have you bothered to read anything describing how Illumina sequencing works?

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All your questions read like homework assignments to me. Please make an effort to read some review articles before asking.

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No, it is not homework assignments, but I am not a biologist and I am new in bioinformatics area and I want to know what does it mean because I am confused about it.

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6.7 years ago

I am not going to directly answer the question that you asked.  However, I am going to answer your question.  

Different fields have different cultures.  Science, and particularly team science fields such as biology, require communication between collaborators and team members.  Everyone brings different expertise to the table and there is an expectation that:

  1. folks share their own expertise
  2. folks ask questions and understand the science in which they are involved

So, as a collaborator (team member), it is important to share your expertise, but it is also at least partially your responsibility to understand the science by asking questions of your collaborators.  Ideally, the scientific process produces a result (manuscript, data, etc.) that will require your signoff.  

I do not want to discourage you from asking questions here on this list, but there is a bit of an expectation that you do so only after exhausting local resources such as your collaborators, team members, and literature/web searches.  Since you are new to biology, you are new to the culture.  Work with your local experts, your sequencing facility or company.  Try to establish a set of goto resources that you can rely on.  When all else fails, the biostars community is here to help.

And, OK, I will answer your question, also:

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