This is my first post and first time using and learning Bioperl for bioinformatics applications.
I have a multiple sequence alignemnt file and I am trying to print out the consensus sequence based for the whole alignement. The problem is how do you run the program to print the consensus sequence. Below I am pasting the code which I got from the Bioperl Wiki page.
Any help will be greatly appreciated. Thank you.
my $aln = Bio::SimpleAlign->new('msa_A_PreS1.aln');
my $threshold_percent = 90;
my $consensus_with_threshold = $aln->consensus_string($threshold_percent);
my $iupac_consensus = $aln->consensus_iupac(); # dna/rna alignments only
my $percent_ident = $aln->percentage_identity;
my $seqname = ''; #'gnl|hbvcds|AB014370_PreS1_P-A';
my $pos = $aln->column_from_residue_number($seqname, 14);
I want to print the whole consensus sequence and not just a specific position or column.