i'm trying to extract the nucleotide distribution, for each position, from a .sam/.bam file ?!
I don't look for the total depth of coverage (that can be done with GATK or samtools), but the depth of coverage for each nucleotide, at each position in my alignment file (bam/sam/...)
How can i do that ?
Do you know a tools that can do that ?
Thanks in advance for any answer/suggestion,