Standalone Blast problem

Question

[Mohit]

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I want to blast the sequences against my own database... So I am using standalone blast utility. while using update_blastdb.pl I have come accross the error of "Failed to connect to ftp.ncbi.nlm.nih.gov: Invalid argument".

Command: perl update_blastdb.pl --passive refseq_rna.00

Error: Failed to connect to ftp.ncbi.nlm.nih.gov: Invalid argument

Why this error?

[Mohit]

yeah by using internet browser I can access this site...

OS--> Windows7 ultimate 64bit

Perl5.16.3

ftp 7.5

Answer 1

[Michael]

As I suspected it's a windows problem with Net::FTP, possibly passive mode or options are not supported by perl or your version of FTP on windows this way. Try without switch --passive or try -passive, otherwise you have to download files manually or try a real operating system.

update_blastdb.pl --passive refseq_rna # use dbname not volume filename (refseq_rna.00)

[Mohit]

Still its not working Same error again!

[Michael]

works for me, maybe something wrong with your internet connection (check if you can access ftp.ncbi.nlm.nih.gov)? otherwise more details please: which operating system (windows prob.?) perl version etc. ftp version, etc, etc.