Question: Standalone Blast problem
0
gravatar for Mohit
5.3 years ago by
Mohit0
India
Mohit0 wrote:

H

I want to blast the sequences against my own database... So I am using standalone blast utility. while using update_blastdb.pl I have come accross the error of "Failed to connect to ftp.ncbi.nlm.nih.gov: Invalid argument". 

Command: perl update_blastdb.pl --passive refseq_rna.00

Error: Failed to connect to ftp.ncbi.nlm.nih.gov: Invalid argument

why this error?

blast forum • 2.1k views
ADD COMMENTlink modified 5.3 years ago • written 5.3 years ago by Mohit0

yeah by using internet browser i can access this site...
OS--> Windows7 ultimate 64bit

Perl5.16.3

ftp 7.5

 

ADD REPLYlink written 5.3 years ago by Mohit0
0
gravatar for Michael Dondrup
5.3 years ago by
Bergen, Norway
Michael Dondrup46k wrote:

As I suspected it's a windows problem with Net::FTP, possibly passive mode or options are not supported by perl or your version of FTP on windows this way. Try without switch '--passive' or try '-passive', otherwise you have to download files manually or try a real operating system.

 

update_blastdb.pl --passive refseq_rna 

# use dbname not volume filename (refseq_rna.00)

ADD COMMENTlink modified 5.3 years ago • written 5.3 years ago by Michael Dondrup46k

Still its not working Same error again!

ADD REPLYlink written 5.3 years ago by Mohit0

works for me, maybe something wrong with your internet connection (check if you can access  ftp.ncbi.nlm.nih.gov)? otherwise more details please: which operating system (windows prob.?) perl version etc. ftp version, etc, etc.

ADD REPLYlink written 5.3 years ago by Michael Dondrup46k
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