Off topic:rtPCR Validated Fusion - How did it happen?
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9.7 years ago
earonesty ▴ 250

I have a RNA fusion event that we confirmed with rtPCR. The distance between the fused exons is 20 million bases, which should rule out a read-through event (correct?).

However, we don't observe unusual coverage or copy number variation in the genome.

Are there other mechanisms you can think of that would explain the presence of this fused product?

fusion RNA-Seq • 1.5k views
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