Hello, I have a data set with expression values (counts). I assembled the transcriptome myself, since there is no reference to map to. I started with 130,000 transcripts (rows on my data table) and brought it down to about 70,000 by filtering out all transcripts with (sum of row FPKM) < 1. This was to eliminate lowly expressed gene and stochastic noise. I don't know how to futher filter my dataset to only find differentially expressed genes, unfortunately. Any tips or resources?
Question: How to filter out an RNA-seq differential expression data set?
4.6 years ago by
pvaysberg • 0
pvaysberg • 0 wrote:
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