Question: PGM reads 3' end trimming
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gravatar for Max Ivon
4.5 years ago by
Max Ivon110
Russian Federation
Max Ivon110 wrote:

Hello!

We use PGM for target sequencing (signle end reading). Can someone explain why some reads are trimmed in the 3' end? I mean at what stage of sequencing it happends. And can be 5' end trimmed? There is no any strand bias of trimming. Reads are trimmed already in fastq file derived straightly from PGM, so it is not effect of any soft or tool.Trimming length differs from read to read, it is not constant.

Thanks in advance!

trimming pgm • 1.4k views
ADD COMMENTlink modified 4.4 years ago by Biostar ♦♦ 20 • written 4.5 years ago by Max Ivon110

What is your question? How you know that reads have been trimmed? 

ADD REPLYlink written 4.4 years ago by Ashutosh Pandey11k
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