plz anyone can tell me the procedure for miRNA identification in animals using EST.. I did blastn and blastx search to select the input for mFOLD program. how could I select the best structure in that result? whether based on refined or initial dG value? Even though I read many articles, I am not clear about how to identify precursors and how to identify mature miRNA?? In all of them they put select the precursors based on 50nt up and down of mature miRNA.. i dn't know to select mature miRNA.. I really fed-up with referring through articles. plz help me. this is my project..