Question: How to use maximum identity > 95%, length > 200 bp as search parameters for tblastn standalone version
0
gravatar for chevivien
3.1 years ago by
chevivien60
China
chevivien60 wrote:

hallo guys,

Could someone please suggest to me how to use following search parameters for tblastn search(stand alone version)

 Search parameters : maximum identity > 95%, length > 200 bp and Evalue < 10e-10.(borrowed idea from http://www.biomedcentral.com/1471-2164/12/76;Digital gene expression section)

iam well conversant with blast commands such as : tblastn -db blastdb/nippest -query mydata.fasta -evalue 10e-10 >out.nippest - outfmt 11.

Following the attached paper i wish to add maximum identity >95% and length 200 bp as part of search parameters but i can'nt figure out  how to go about. I have checked all NCBI BLAST documentations  but i cannt find the solution.

Thanks

ADD COMMENTlink modified 3.1 years ago • written 3.1 years ago by chevivien60

seems duplicate of your own previous question tblastn search parameters 

ADD REPLYlink modified 3.1 years ago • written 3.1 years ago by Prakki Rama2.0k

Yes,its...i wrote the first question by mistake on the comment section of another question i had asked earlier on (gene duplication) by mistake and couldn't  find a way to delete it....

.That's why i posted it as new post But i have figured out how to delete it.Thanks.

ADD REPLYlink modified 3.1 years ago • written 3.1 years ago by chevivien60
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