Question: Are there any "complete" VCF files for tomato available?
0
gravatar for gabriel.jabud
4.7 years ago by
United States
gabriel.jabud0 wrote:

I'm following the GATK pipeline for variant calling of tomato whole genome data I am analyzing and am stuck on the base recalibration step (http://www.broadinstitute.org/gatk/guide/best-practices?bpm=DNAseq).  It requires me to use a VCF file containing known variant sites.  The only VCF file I've found so far is the one on NCBI's dbSNP database (ftp://ftp.ncbi.nlm.nih.gov/snp/organisms/tomato_4081/VCF/).  However the combined VCF file for all 12 chromosomes is only about 237 lines long including the header, so I doubt it will help to recalibrate with such a small amount of known variants.

Should I just go ahead and skip the recalibration step or is there a more complete VCF file containing variants of tomato?

gatk solanum variants tomato vcf • 1.5k views
ADD COMMENTlink written 4.7 years ago by gabriel.jabud0

You'll likely need to bootstrap.

ADD REPLYlink written 4.7 years ago by Devon Ryan89k
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