ANIb versus ANIm
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Entering edit mode
7.4 years ago
njs531 ▴ 20

Hi,

I'm conducting a research to explore bacterial diversity using R. leguminorarum population as a model. I'm using Average nucleotide identity (ANI) analysis to delineate the species in this population. I'm not too sure whether to use ANIb or ANIm so I have chosen ANIb which is the default option. I've read that ANIm is used to decrease the running time . Besides that, is there a significant difference between the two parametersĀ  andĀ  is my result going to be less accurate if I use ANIb instead of ANIm?

Bacteria ANIb ANIm Average-Nucleotide-Identity • 4.1k views
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Entering edit mode
7.4 years ago
cts ★ 1.7k

Check out this paper for a comparison of the two methods as well as a third way of performing these calculations using tetranucleotide frequencies.

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