I have been trying to assemble bacterial genome generatad by illumina Miseq (pair-ends), i have used different assemlers on Galaxy (Velvet, MIRA, etc) but none is working for me. They always come up with errors, both de novo and mapping.
Example of such error is described below with Bowtie.
Please i need help. I believe nothing is wrong with my fastq files because i have used that files with A5 miseq and CLC genomics assemblers and they both worked.
An error occurred with this dataset: Settings: Output files: "/tmp/tmpy1e8C5/tmp4v1BVI.*.ebwt" Line rate: 6 (line is 64 bytes) Lines per side: 1 (side is 64 bytes) Offset rate: 5 (one in 32) FTable chars: 10 Strings: unpacked Max bucket size: default Max bucket size, sqrt mu