data mining: find expression level of a specific gene in specific tisse
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9.7 years ago
Whetting ★ 1.6k

Hi,

I am trying to figure out whether my gene of interest is expressed differentially between two tissues. I have been looking at GEO, but I am not clear on how to actually find my gene. I have tried all possible names I can come up with, but GEO does not seem to know the gene.

I guess this leaves me with the following questions:

  1. Can I search GEO based on chromosome location?
  2. Do you know of other databases that would allow me to do this?
  3. Should I download the raw reads and redo the analysis?
  4. other ideas?

As always, thanks for any help!

database GEO RNA-Seq • 2.0k views
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I always download .sra files and redo the analysis, in this way I know the quality, how reads are mapped and moreover, how the expression is estimated.

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9.7 years ago
Adrian ▴ 700

Are you looking at a particular dataset in GEO? More specific details would be helpful.

You might want to have a look at GTEX, which is a large systematic dataset of gene expression and eQTLs across many human tissues: http://www.gtexportal.org/home/

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