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• Methodological process for GO functional annotation of my positively selected candidate genes
  I did my homework first here and elsewhere but still find it difficult to fit pieces of informati...
• Priority Of Blast Hits In Blast2Go
  Hi all, I'm using b2g4pipe for annotation of genomes. The first step is run blastx against some ...
• Gene Ontology Term Generation For Keywords
  Hi to all, I have found the most probable function for sequences using HMM methods. These sequen...
• Functional annotation(GO,KEGG): Blast2GO+KAAS or EggNOG
  I have a non-model organism and I tried to use two different strategies to do functional annotati...
• How to look up GO terms in BioMart using GI ID or Accession ID
  Hi, I am working on a research project where I need to add Gene Ontology terms for each hits fro...
• Direct vs Indirect annotations of a term in GO (Gene Ontology) Database
  Reference to the [Paper](https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=7454750&amp...
• Retrieve Go Terms Using Uniprot Blasts Results (Together W/ Gene_Association.Goa_Uniprot.Gz)
  Hi all! I got some differential expressed (DE) genes from a non-model RNA-seq project and I'd l...
• Ways to merge GO terms from different analyses?
  Hello! I would like to do gene ontology annotation of a set of proteins from a non-model organis...
• Find the best hit of heterologous protein in blastx results
  Hello! Lets take the story from the beginning. I am running a blastx for the query (contig) abov...
• Super Transcripts approach
  Hi all! Have any of you used Corset (https://github.com/Oshlack/Corset/wiki) and Lace programs ...
• Gene Ontology enrichment
  Can anyone tell me what tools should I use in gene ontology analysis. David or  Ontologizer.jar. ...
• Gene Ontology: Disjoint Terms
  Hi all, I recently started working with the [Gene Ontology][1] (GO), a database which allows to ...
• Going from BlastX to GO annotation
  Dear all, I have manually created a customized database on NCBI+ tools on a command line centOS ...
• Finding gene ontology terms for a list of genes
  Hello, I have done a gene enrichment analysis for a list of 2000 genes using PANTHER where 300 g...
• an introduction to GO annotations
  I have the mouse genome split into 1000 bp bins. For each bin, I'd like to know how far is it fro...
• Gene Ontology Like Term Clustering
  Hello All, I'm looking for a tool which can cluster Gene Ontology (GO) like terms clustering. Th...
• Gene ontology enrichment non model organism (doubts about the methods)
  Hello community, like many others newbies in Bioinformatics, I have found a plethora of methods ...
• blastx output annotation
  Hi guys, I am wondering if there is an easy way to annotate the output of the blastx (Protein ...
• For multiple testing correction in GO analysis, is it ok to remove GO terms with only 1 gene hit?
  I am doing a GO analysis for my gene sets and plan to implement the Benjamini-Hochberg method to ...
• GO terms : Ensembl ID / enrichment analysis
  Hi I have two lists of human genes. I used FatiGO for enrichment analysis FatiGO takes two lists...
• B2G4Pipe (Command-Line Blast2Go) Question About Output / Throughput
  I am new to BLAST2GO in general, and am using b2g4pipe, and am puzzled by the output. Background...
• Selecting non-redundant Gene Ontology terms
  Hi, I am trying to assign Gene Ontology (GO) Biological Process terms to proteins so that I can d...