Question: Recommended Tools For Isoform Expresion From ExonArrays
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gravatar for Ice P
6.2 years ago by
Ice P0
United States
Ice P0 wrote:

I'm new to this field. I working on Exonarray data and want to get isoform expressions from CEL files, for further analysis. Could you suggest some tools, preferably in R or other open source. I have tried mmbgx in R but it is very time consuming. How can i be assure that the expression results are good. Is a comparison with RT-PCR results a good choice? 

 

 

R isoforms exon-arrays • 1.3k views
ADD COMMENTlink modified 6.2 years ago by dario.garvan480 • written 6.2 years ago by Ice P0
1
gravatar for dario.garvan
6.2 years ago by
dario.garvan480
Australia
dario.garvan480 wrote:

The FIRMA algorithm was designed for such an analysis. The method has been published in a peer-reviewed journal and software is available to use. I am sure if you wanted to publish your findings, you would have to choose a few genes and do qPCR validation of them.

ADD COMMENTlink written 6.2 years ago by dario.garvan480
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