**2.8k**wrote:

Here is an worked out example of calculation of Normalized intensity ratio for i-TRAQ reporter ions by a commercial software Scaffold Q+

*The reporter ion intensities from all the identified peptide spectra are used to calculate non-normalized reporter ion ratios. These ratios are then used to compute the media adjusted normalized intensities. For example, all the reporter ion intensities are multiplied by their respective median non-normalized ratio. Once these median adjusted normalized intensities are calculated, new ratios are computed*

Though the rest of the steps are obvious, I'm not sure how the "Median adjusted Normalized Intensity" values are calculated at the second step utilizing Non-normalized ratios. Can anybody please elaborate?