Here is an worked out example of calculation of Normalized intensity ratio for i-TRAQ reporter ions by a commercial software Scaffold Q+
The reporter ion intensities from all the identified peptide spectra are used to calculate non-normalized reporter ion ratios. These ratios are then used to compute the media adjusted normalized intensities. For example, all the reporter ion intensities are multiplied by their respective median non-normalized ratio. Once these median adjusted normalized intensities are calculated, new ratios are computed
Though the rest of the steps are obvious, I'm not sure how the "Median adjusted Normalized Intensity" values are calculated at the second step utilizing Non-normalized ratios. Can anybody please elaborate?