I am trying to sequence a plasmid, but I get some weird results and it seems that a region has been duplicated.
Now I am wondering: what approach might be the best in order to try and sequence in completely? Or is it pointless?
Now I have 2 contigs and by using primer walking I tried to extent the contigs in order to link them, but what happens is that rather than extending it, the new sequences are just "placed" inside the other contig or they are added to the other side of the contig. It does not extent the contig!
It is pretty weird.
Its sanger sequencing, 1 plasmid.