I want to create a database for local blast but fasta file with reads is 9Gb. So I want from this file to create only one database for the blast and not many files. So I put
-max_file_sz argument as you can see above, but it creates many files of 2.1GB. How can i create only one database file? Should I merge them later?
Here is the command I use.
makeblastdb -in /mnt/usb/sra/merged.fasta -max_file_sz '10GB' -dbtype nucl -out /mnt/usb/sra/merged_db
Thank you but this doesn't really merge the databases. Instead, it creates a virtual database in a .nal file.
So is there any way to merge .nhr , .nin and .nsq files to have a single only database?