I want more details about Strand-specific vs. Non strand-specific protocols in RNAseq. I know that the benefit of strand-specific is to know whether the read originated from the +ve or - ve strand, also it helps identify antisense RNA, and I know it costs more than non-strand-specific. So could you please give me more information about this protocol. I am going to do RNA-seq analysis for human and mouse to detect antisense.