I want more details about Strand-specific vs. Non strand-specific protocols in RNAseq. I know that the benefit of strand-specific is to know whether the read originated from the +ve or - ve strand, also it helps identify antisense RNA, and I know it costs more than non-strand-specific. So could you please give me more information about this protocol. I am going to do RNA-seq analysis for human and mouse to detect antisense.
The strand specific library should only be slightly more expensive than the non-strand specific library from what I remember. So I always prefer the stranded library. What kind of information do you want? The procedure of the protocol? Or the type of strand specific library?
You might want to read this discussion
Could you please send me the information about the procedure of the protocol because I am not biologist major and I want to know more about it. Also, about the type of strand specific library.
BTW, the discussion link doesn't work
The link works for me.