Hi bioinfo guys,
I am new in the field of DNA methylation. I read one paper where they have described about the methylation level of Cytosine in DNA. I am working on bench and for me either the C is methylation or not but how it could be 80% methylation or 50% methylation. I know the procedure that this methylation level came from the aligned reads which are methylation / total alinged read. But I really would like to know What does this actually meant for? Is it related with the probability of occurance of methylation at that C?
I would appreciate if somebody could explain this to me in detail.
Thanks in advance.