I have sequenced several samples on Ilumina Miseq, generating paired-end reads (2*250bp) spanning V3-V4 region of 16S rDNA.
I want to analyze these samples with QIIME v1.8. The script
split_libraries_fastq.py in QIIME aimes to demultiplex and quality filter raw fastq seqeunces, with seperate fastq files for sequence and barcode reads as input.
However, at present I only have demultiplexed paired-end fastq files produced by Miseq, which were generated by Miseq by default when sequencing was completed. And I don't have the barcode fastq files required by QIIME. What should I do to proceed with
split_libraries_fastq.py to process my demultiplexed files?