I have a question about restriction enzymes and their use in helping to ID CRISPR induced mutations. Once a CRISPR causes an indel at a target sequence, a restriction enzyme can be used to screen several individuals exposed to the CRISPR, screening them for signs of an indel. I am not clear on a point related to this. Do I need to choose a restriction enzyme that binds to the area where the CRISPR induces a mutation? Or do I need to choose a restriction enzyme that cuts in the area where the CRISPR induces a mutation?
Question: (Closed) restriction digest to test for CRISPR disruption
5.6 years ago by
es • 30
es • 30 wrote:
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