Question: Could the information of Line 3 in fastq file be deleted?
1
gravatar for Cacau
4.9 years ago by
Cacau450
Canada
Cacau450 wrote:

It seems that the third line of a sequence in a fastq formatted file may not matter. Could I just keep a '+' for Line 3?

rna-seq next-gen • 1.5k views
ADD COMMENTlink modified 4.9 years ago by Peter5.8k • written 4.9 years ago by Cacau450
2
gravatar for Peter
4.9 years ago by
Peter5.8k
Scotland, UK
Peter5.8k wrote:

Yes, you can, and in fact I would say you should remove the optional redundant information from the plus line. This is recommended to save space http://dx.doi.org/10.1093/nar/gkp1137

ADD COMMENTlink written 4.9 years ago by Peter5.8k
1
gravatar for Renesh
4.9 years ago by
Renesh1.6k
United States
Renesh1.6k wrote:

Yes you can do that to reduce the size of file.

ADD COMMENTlink written 4.9 years ago by Renesh1.6k

Do not delete 3rd line in fastq file, because most (all) of the tools accepts standard format i.e four lines for each record

ADD REPLYlink written 4.9 years ago by Renesh1.6k

Thanks! So to keep a '+' is OK for Line 3?

ADD REPLYlink written 4.9 years ago by Cacau450

Yes, you just need the plus sign, the remainder isn't needed.

ADD REPLYlink written 4.9 years ago by Devon Ryan91k

Thanks a lot!

ADD REPLYlink written 4.9 years ago by Cacau450
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 852 users visited in the last hour