Could the information of Line 3 in fastq file be deleted?
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10.1 years ago
Cacau ▴ 520

It seems that the third line of a sequence in a fastq formatted file may not matter. Could I just keep a '+' for Line 3?

next-gen RNA-Seq • 3.2k views
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10.1 years ago
Peter 6.0k

Yes, you can, and in fact I would say you should remove the optional redundant information from the plus line. This is recommended to save space http://dx.doi.org/10.1093/nar/gkp1137

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10.1 years ago
Renesh ★ 2.2k

Yes you can do that to reduce the size of file.

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Do not delete 3rd line in fastq file, because most (all) of the tools accepts standard format i.e four lines for each record

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Thanks! So to keep a '+' is OK for Line 3?

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Yes, you just need the plus sign, the remainder isn't needed.

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Thanks a lot!

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