So I have a full genome PACbio BAM file and this has a average coverage of 3-6.
Now I want to split the bam file per read. Since readgroup is not working and sometimes still gives me multiple reads inside my BAM.
The reason why I want this is because i want to run MPILEUP with only 1 read per bam, so that I can identify insertions/deletions per read on a given location. Anyone knows a program to do this? Or another approach to do it?